ArticleTissue engineering. Part A2015
Combination of BMP2 and MSCs significantly increases bone formation in the rat arterio-venous loop model.
Article in Tissue engineering. Part A, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
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- External Screw-Threaded Traction Device Helps Optimize Finger Joint Mobility in Severe Stage III and IV Dupuytren Disease.Medical science monitor : international medical journal of experimental and clinical research · 2021Article
- BMP2 induces hMSC osteogenesis and matrix remodeling.Molecular medicine reports · 2021Article
- Bone tissue engineering using adipose-derived stem cells and endothelial cells: Effects of the cell ratio.Journal of cellular and molecular medicine · 2020Article
- Reconstruction of segmental mandibular defects: Current procedures and perspectives.Laryngoscope investigative otolaryngology · 2019Review
- Material-Induced Venosome-Supported Bone Tubes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- A Low Cost Implantation Model in the Rat That Allows a Spatial Assessment of Angiogenesis.Frontiers in bioengineering and biotechnology · 2018Article
- The Arteriovenous (AV) Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering.Journal of visualized experiments : JoVE · 2016Article
- Bone Graft Prefabrication Following the In Vivo Bioreactor Principle.EBioMedicine · 2016Review
- Comment on 'Basic concepts regarding fractures healing and the current options and future directions in managing bone fractures'.International wound journal · 2016Article
- Distal Row Carpectomy-A Possible Salvage Procedure of Severe Carpal Trauma.Plastic and reconstructive surgery. Global open · 2015Article
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Authors and funding
11 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
introductionIn this study the induction of bone formation in an axially vascularized bone matrix using mesenchymal stem cells (MSCs) and application of bone morphogenetic protein 2 (BMP2) was analyzed in the arteriovenous loop (AVL) model. MATERIALS AND
methodsAn AVL was created in the medial thigh of 42 rats and placed in a porous titanium chamber filled with a particulated porous hydroxyapatite and beta-tricalcium phosphate matrix and fibrin. In group A the fibrin was loaded with 5×10(6) DiI-stained fibrin gel-immobilized primary MSCs from syngenic Lewis rats, in group B the matrix was loaded with 60 μg/mL BMP2 and in group C both, BMP2 and MSCs were applied at implantation time point. After 6 and 12 weeks, specimens were investigated by means of histological, morphometrical, and micro-computed tomography analysis.
resultsAfter implantation of an AVL a dense vascular network was visible in all groups. In group A, newly generated bone islands were detected in the periphery of the main vascular axis. Using BMP2 alone (group B), small islands of newly formed bone were visible evenly distributed in all parts of the constructs. In group C nearly the whole matrix was interspersed with bone formations. In all groups there was an increase of bone formation between the 6 and 12 weeks explantation time points.
conclusionsThis study demonstrates for the first time the successful generation of axially vascularized bone substitutes using MSCs and BMP2 in the AVL rat model using a one step procedure. Using the combination of BMP2 and MSCs there was a significant increase of bone formations detectable compared to the BMP2 or MSCs alone groups.
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