ArticleJournal of biomedical optics2014
Second harmonic generation microscopy analysis of extracellular matrix changes in human idiopathic pulmonary fibrosis.
Article in Journal of biomedical optics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 70 citations in OpenAlex.
- Label-free, multiphoton microscopy-based strategy for intraoperative grading of lung adenocarcinoma via quantitative extracellular matrix analysis.Biomedical optics express · 2026Article
- Artificial Second Harmonic Generation Image Feature Tuning with Generative Models and SeFa Semantic Analysis.JPhys photonics · 2026Article
- Quantitative label-free multiphoton imaging of collagen features predicts disease severity and progression in idiopathic pulmonary fibrosis.Respiratory research · 2026Observational
- Deep Learning Framework for Early Detection of Pancreatic Cancer Using Multi-modal Medical Imaging Analysis.Journal of imaging informatics in medicine · 2026Article
- Generalized 3D quasi-phase-matching model of image contrast in second harmonic generation microscopy of fibrillar collagen architectures.JPhys photonics · 2026Article
- Imaging the extracellular matrix structure and remodeling in healthy, fibrotic, and emphysematous human precision-cut lung slices.Respiratory research · 2025Article
- Review
- Mechanisms on How Matricellular Microenvironments Sustain Idiopathic Pulmonary Fibrosis.International journal of molecular sciences · 2025Review
- Classification of collagen remodeling in asthma using second-harmonic generation imaging, supervised machine learning and texture-based analysis.Frontiers in bioinformatics · 2025Article
- Phasor-FLIM and SHG imaging for quantitative analysis of lung cancer autofluorescence.Computational and structural biotechnology journal · 2025Article
- Proteolysis and Contractility Regulate Tissue Opening and Wound Healing by Lung Fibroblasts in 3D Microenvironments.Advanced healthcare materials · 2024Article
- Towards next-generation diagnostic pathology: AI-empowered label-free multiphoton microscopy.Light, science & applications · 2024Review
- Fourier transform-based method for quantifying the three-dimensional orientation distribution of fibrous units.Scientific reports · 2024Article
- Article
- Imaging of colorectal adenomas with pseudoinvasion and malignant polyps using two-photon excitation microscopy.Frontiers in oncology · 2024Article
- Lung extracellular matrix modulates KRT5Nature communications · 2023Article
- Effect of tissue fixation on the optical properties of structural components assessed by non-linear microscopy imaging.Biomedical optics express · 2023Article
- Don't go breakin' my heart: cardioprotective alterations to the mechanical and structural properties of reperfused myocardium during post-infarction inflammation.Biophysical reviews · 2023Review
- PSHG-TISS: A collection of polarization-resolved second harmonic generation microscopy images of fixed tissues.Scientific data · 2022Article
- Analysis of age-related left ventricular collagen remodeling in living donors: Implications in arrhythmogenesis.iScience · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Patients with idiopathic fibrosis (IPF) have poor long-term survival as there are limited diagnostic/prognostic tools or successful therapies. Remodeling of the extracellular matrix (ECM) has been implicated in IPF progression; however, the structural consequences on the collagen architecture have not received considerable attention. Here, we demonstrate that second harmonic generation (SHG) and multiphoton fluorescence microscopy can quantitatively differentiate normal and IPF human tissues. For SHG analysis, we developed a classifier based on wavelet transforms, principle component analysis, and a K-nearest-neighbor algorithm to classify the specific alterations of the collagen structure observed in IPF tissues. The resulting ROC curves obtained by varying the numbers of principal components and nearest neighbors yielded accuracies of >95%. In contrast, simpler metrics based on SHG intensity and collagen coverage in the image provided little or no discrimination. We also characterized the change in the elastin/collagen balance by simultaneously measuring the elastin autofluorescence and SHG intensities and found that the IPF tissues were less elastic relative to collagen. This is consistent with known mechanical consequences of the disease. Understanding ECM remodeling in IPF via nonlinear optical microscopy may enhance our ability to differentiate patients with rapid and slow progression and, thus, provide better prognostic information.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.