ArticleAnalytical chemistry2014
MALDI-guided SIMS: multiscale imaging of metabolites in bacterial biofilms.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Molecular imaging of bacterial biofilms-a systematic review.Critical reviews in microbiology · 2024Pooled it
- Computational Mass Spectrometry Imaging in the Era of AI.Chemical reviews · 2026Review
- UnderstandingMicroorganisms · 2026Review
- Mass Spectrometry Imaging.Analytical chemistry · 2025Review
- Use of analytical strategies to understand spatial chemical variation in bacterial surface communities.Journal of bacteriology · 2025Review
- Article
- Frontiers in Mass Spectrometry-Based Spatial Metabolomics: Current Applications and Challenges in the Context of Biomedical Research.Trends in analytical chemistry : TRAC · 2024Article
- Matrix-assisted laser desorption/ionization mass spectrometry imaging for quorum sensing.AMB Express · 2024Review
- Metabolic insights from mass spectrometry imaging of biofilms: A perspective from model microorganisms.Methods (San Diego, Calif.) · 2024Review
- Combating multidrug-resistance inNAR molecular medicine · 2024Article
- Toward Comprehensive Analysis of the 3D Chemistry ofAnalytical chemistry · 2023Article
- Simultaneous spatiotemporal transcriptomics and microscopy of Bacillus subtilis swarm development reveal cooperation across generations.Nature microbiology · 2023Article
- Chemical Imaging of Pharmaceuticals in Biofilms for Wastewater Treatment Using Secondary Ion Mass Spectrometry.Environmental science & technology · 2023Article
- Probing Interkingdom Signaling Molecules via Liquid Extraction Surface Analysis-Mass Spectrometry.Analytical chemistry · 2023Article
- Comparison of Small Biomolecule Ionization and Fragmentation inJournal of the American Society for Mass Spectrometry · 2023Article
- Label-free sub-micrometer 3D imaging of ciprofloxacin in native-state biofilms with cryo-time-of-flight secondary ion mass spectrometry.Analytical and bioanalytical chemistry · 2023Article
- Advances in Mass Spectrometry-Metabolomics Based Approaches.Advances in experimental medicine and biology · 2023Article
- Reducing the matrix effect in mass spectral imaging of biofilms using flow-cell culture.Frontiers in chemistry · 2023Article
- Review
- MALDI Mass Spectrometry Imaging Reveals the Existence of anMetabolites · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Mass spectrometry imaging (MSI) is a versatile tool for visualizing molecular distributions in complex biological specimens, but locating microscopic chemical features of interest can be challenging in samples that lack a well-defined anatomy. To address this issue, we developed a correlated imaging approach that begins with performing matrix-assisted laser desorption/ionization (MALDI) MSI to obtain low-resolution molecular maps of a sample. The resulting maps are then used to direct subsequent microscopic secondary ion mass spectrometry (SIMS) imaging and tandem mass spectrometry (MS/MS) experiments to examine selected chemical regions of interest. By employing MALDI undersampling, the sample surface is left mostly unperturbed and available for the SIMS analysis, while also generating an ablation array that can be used for navigation in SIMS. We validated this MALDI-guided SIMS approach using cultured biofilms of the opportunistic pathogen Pseudomonas aeruginosa; bioactive secondary metabolites, including rhamnolipids and quinolones, were detected and visualized on both macro- and microscopic size scales. MSI mass assignments were confirmed with in situ MALDI MS/MS and capillary electrophoresis-electrospray ionization MS/MS analysis of biofilm extracts. Two strains of P. aeruginosa were compared, wild type and a quorum sensing mutant, and differences in metabolite abundance and distribution were observed.
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Registered trials
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