ArticlePloS one2014
High throughput micro-well generation of hepatocyte micro-aggregates for tissue engineering.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Micro Pattern-Based 3D Cell Culture Platform: An Overview of Technologies and Applications.Exploration (Beijing, China) · 2026Review
- Review
- Engineered 3D liver-tissue model with minispheroids formed by a bioprinting process supported withBioactive materials · 2024Article
- Construction and applications of the EOMA spheroid model of Kaposiform hemangioendothelioma.Journal of biological engineering · 2024Article
- The 3Rs in Experimental Liver Disease.Animals : an open access journal from MDPI · 2023Review
- Preclinical Models and Promising Pharmacotherapeutic Strategies in Liver Fibrosis: An Update.Current issues in molecular biology · 2023Review
- Chondrogenesis of Adipose-Derived Stem Cells Using an Arrayed Spheroid Format.Cellular and molecular bioengineering · 2022Article
- Fabrication of Concave Microwells and Their Applications in Micro-Tissue Engineering: A Review.Micromachines · 2022Review
- Article
- SpheroidChip: Patterned Agarose Microwell Compartments Harboring HepG2 Spheroids are Compatible with Genotoxicity Testing.ACS biomaterials science & engineering · 2020Article
- Bioprinting predifferentiated adipose-derived mesenchymal stem cell spheroids with methacrylated gelatin ink for adipose tissue engineering.Journal of materials science. Materials in medicine · 2020Article
- Hybrid Bioprinting of Chondrogenically Induced Human Mesenchymal Stem Cell Spheroids.Frontiers in bioengineering and biotechnology · 2020Article
- A simple method for the generation of insulin producing cells from bone marrow mesenchymal stem cells.In vitro cellular & developmental biology. Animal · 2019Article
- High-throughput image-based monitoring of cell aggregation and microspheroid formation.PloS one · 2018Article
- The Self-Assembling Process and Applications in Tissue Engineering.Cold Spring Harbor perspectives in medicine · 2017Review
- Controlled Self-assembly of Stem Cell Aggregates Instructs Pluripotency and Lineage Bias.Scientific reports · 2017Article
- Multiscale cytometry and regulation of 3D cell cultures on a chip.Nature communications · 2017Article
- One step fabrication of hydrogel microcapsules with hollow core for assembly and cultivation of hepatocyte spheroids.Acta biomaterialia · 2017Article
- Cell sources for in vitro human liver cell culture models.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair.Trends in biotechnology · 2016Review
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The main challenge in hepatic tissue engineering is the fast dedifferentiation of primary hepatocytes in vitro. One successful approach to maintain hepatocyte phenotype on the longer term is the cultivation of cells as aggregates. This paper demonstrates the use of an agarose micro-well chip for the high throughput generation of hepatocyte aggregates, uniform in size. In our study we observed that aggregation of hepatocytes had a beneficial effect on the expression of certain hepatocyte specific markers. Moreover we observed that the beneficial effect was dependent on the aggregate dimensions, indicating that aggregate parameters should be carefully considered. In a second part of the study, the selected aggregates were immobilized by encapsulation in methacrylamide-modified gelatin. Phenotype evaluations revealed that a stable hepatocyte phenotype could be maintained during 21 days when encapsulated in the hydrogel. In conclusion we have demonstrated the beneficial use of micro-well chips for hepatocyte aggregation and the size-dependent effects on hepatocyte phenotype. We also pointed out that methacrylamide-modified gelatin is suitable for the encapsulation of these aggregates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.