ArticleThe Journal of clinical investigation2014
PAX7 expression defines germline stem cells in the adult testis.
Article in The Journal of clinical investigation, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 172 citations in OpenAlex.
- IL1R2 Marks and Sorts a Spermatogonial Stem Cell Population Critical for Restoring Spermatogenesis Upon Interruption in Mammals.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Intrinsic dysfunction in muscle stem cells lacking dystrophin begins during secondary myogenesis.Nature communications · 2025Article
- Interdomain disulfide bonds of rabbit kappa light chain allotypes influence mouse-rabbit chimeric antibody performance.bioRxiv : the preprint server for biology · 2025Article
- A convenient research strategy for functional verification of epigenetic regulators during spermatogenesis.Asian journal of andrology · 2025Article
- The Biology and Regulation of Spermatogonial Stem Cells in the Niche.Advances in experimental medicine and biology · 2025Review
- Isolation of Germ Cell Populations from Mouse Testis by Flow Cytometry.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Single-cell transcriptome analyses reveal critical regulators of spermatogonial stem cell fate transitions.BMC genomics · 2024Article
- Identification of quiescent FOXC2eLife · 2023Article
- Decoding the spermatogonial stem cell niche under physiological and recovery conditions in adult mice and humans.Science advances · 2023Article
- Cell type-specific role of CBX2 and its disordered region in spermatogenesis.Genes & development · 2023Article
- Branched germline cysts and female-specific cyst fragmentation facilitate oocyte determination in mice.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Defining Gene Function in Spermatogonial Stem Cells Through Conditional Knockout Approaches.Methods in molecular biology (Clifton, N.J.) · 2023Article
- In Vivo Investigation of Gene Function in Muscle Stem Cells by CRISPR/Cas9-Mediated Genome Editing.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Transcription and chromatin regulation by TAF4b during cellular quiescence of developing prospermatogonia.Frontiers in cell and developmental biology · 2023Article
- Lineage Tracing of Spermatogonial Stem Cells Within the Male Germline.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Differentially expressed genes in the testes from early to mature development of banana shrimp (Fenneropenaeus merguiensis).PloS one · 2023Article
- Perspectives: Approaches for Studying Livestock Spermatogonia.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Distinctive molecular features of regenerative stem cells in the damaged male germline.Nature communications · 2022Article
- Liposome-Encapsulated Rec8 and Dmrt1 Plasmids Induce Red-Spotted Grouper (Epinephelus akaara) Testis Maturation.Marine biotechnology (New York, N.Y.) · 2022Article
- Developmental alterations in DNA methylation during gametogenesis from primordial germ cells to sperm.iScience · 2022Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
Abstract
Spermatogenesis is a complex, multistep process that maintains male fertility and is sustained by rare germline stem cells. Spermatogenic progression begins with spermatogonia, populations of which express distinct markers. The identity of the spermatogonial stem cell population in the undisturbed testis is controversial due to a lack of reliable and specific markers. Here we identified the transcription factor PAX7 as a specific marker of a rare subpopulation of A(single) spermatogonia in mice. PAX7+ cells were present in the testis at birth. Compared with the adult testis, PAX7+ cells constituted a much higher percentage of neonatal germ cells. Lineage tracing in healthy adult mice revealed that PAX7+ spermatogonia self-maintained and produced expanding clones that gave rise to mature spermatozoa. Interestingly, in mice subjected to chemotherapy and radiotherapy, both of which damage the vast majority of germ cells and can result in sterility, PAX7+ spermatogonia selectively survived, and their subsequent expansion contributed to the recovery of spermatogenesis. Finally, PAX7+ spermatogonia were present in the testes of a diverse set of mammals. Our data indicate that the PAX7+ subset of A(single) spermatogonia functions as robust testis stem cells that maintain fertility in normal spermatogenesis in healthy mice and mediate recovery after severe germline injury, such as occurs after cancer therapy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.