ArticleThe Journal of cell biology1989
Dynamic cytoskeleton-integrin associations induced by cell binding to immobilized fibronectin.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 97 citations in OpenAlex.
- Development of Nascent Focal Adhesions in Spreading Cells.Biophysical journal · 2020Review
- Protein Kinase D1 regulates focal adhesion dynamics and cell adhesion through Phosphatidylinositol-4-phosphate 5-kinase type-l γ.Scientific reports · 2016Article
- Myofibrillar architecture in engineered cardiac myocytes.Circulation research · 2008Article
- Integrins can directly mediate metastatic tumor cell adhesion within the liver sinusoids.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2004Article
- Aberrant activation of focal adhesion proteins mediates fibrillar amyloid beta-induced neuronal dystrophy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Truncation of the cytoplasmic domain of beta3 in a variant form of Glanzmann thrombasthenia abrogates signaling through the integrin alpha(IIb)beta3 complex.The Journal of clinical investigation · 1997Article
- Characterization of cell-matrix adhesion requirements for the formation of fascin microspikes.Molecular biology of the cell · 1997Article
- Integrin alpha 3 beta 1 participates in the phagocytosis of extracellular matrix molecules by human breast cancer cells.Molecular biology of the cell · 1996Article
- The differential adhesion forces of anterior cruciate and medial collateral ligament fibroblasts: effects of tropomodulin, talin, vinculin, and alpha-actinin.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Talin requires beta-integrin, but not vinculin, for its assembly into focal adhesion-like structures in the nematode Caenorhabditis elegans.Molecular biology of the cell · 1996Article
- Integrin function: molecular hierarchies of cytoskeletal and signaling molecules.The Journal of cell biology · 1995Article
- Specialized functional properties of the integrin alpha 4 cytoplasmic domain.Molecular biology of the cell · 1995Article
- Mapping in vivo associations of cytoplasmic proteins with integrin beta 1 cytoplasmic domain mutants.Molecular biology of the cell · 1995Article
- Membrane proteases as potential diagnostic and therapeutic targets for breast malignancy.Breast cancer research and treatment · 1994Review
- Focal contact assembly through cytoskeletal polymerization: steady state analysis.Journal of mathematical biology · 1994Article
- Integrin-cytoskeletal interactions in migrating fibroblasts are dynamic, asymmetric, and regulated.The Journal of cell biology · 1993Article
- Loss of VLA-2 collagen receptor in breast carcinoma, facilitating invasion and metastasis.Japanese journal of cancer research : Gann · 1993Article
- A theoretical analysis for the effect of focal contact formation on cell-substrate attachment strength.Biophysical journal · 1993Article
- Tyrosine phosphorylation of membrane proteins mediates cellular invasion by transformed cells.The Journal of cell biology · 1992Article
- Regulation of the VLA integrin-ligand interactions through the beta 1 subunit.The Journal of cell biology · 1992Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have examined the early events of cellular attachment and spreading (10-30 min) by allowing chick embryonic fibroblasts transformed by Rous sarcoma virus to interact with fibronectin immobilized on matrix beads. The binding activity of cells to fibronectin beads was sensitive to both the mAb JG22E and the GRGDS peptide, which inhibit the interaction between integrin and fibronectin. The precise distribution of cytoskeleton components and integrin was determined by immunocytochemistry of frozen thin sections. In suspended cells, the distribution of talin was diffuse in the cytoplasm and integrin was localized at the cell surface. Within 10 min after binding of cells and fibronectin beads at 22 degrees C or 37 degrees C, integrin and talin aggregated at the membrane adjacent to the site of bead attachment. In addition, an internal pool of integrin-positive vesicles accumulated. The mAb ES238 directed against the extracellular domain of the avian beta 1 integrin subunit, when coupled to beads, also induced the aggregation of talin at the membrane, whereas ES186 directed against the intracellular domain of the beta 1 integrin subunit did not. Cells attached and spread on Con A beads, but neither integrin nor talin aggregated at the membrane. After 30 min, when many of the cells were at a more advanced stage of spreading around beads or phagocytosing beads, alpha-actinin and actin, but not vinculin, form distinctive aggregates at sites along membranes associated with either fibronectin or Con A beads. Normal cells also rapidly formed aggregates of integrin and talin after binding to immobilized fibronectin in a manner that was similar to the transformed cells, suggesting that the aggregation process is not dependent upon activity of the pp60v-src tyrosine kinase. Thus, the binding of cells to immobilized fibronectin caused integrin-talin coaggregation at the sites of membrane-ECM contact, which can initiate the cytoskeletal events necessary for cell adhesion and spreading.
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