ArticleTheScientificWorldJournal2014
Plasminogen activator inhibitor-1 regulates LPS induced inflammation in rat macrophages through autophagy activation.
Article in TheScientificWorldJournal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Functionally integrating nanoparticles alleviate deep vein thrombosis in pregnancy and rescue intrauterine growth restriction.Nature communications · 2022Article
- Extracellular Vesicles From LPS-Treated Macrophages Aggravate Smooth Muscle Cell Calcification by Propagating Inflammation and Oxidative Stress.Frontiers in cell and developmental biology · 2022Article
- Article
- Bioinformatics and Network Pharmacology-Based Approaches to Explore the Potential Mechanism of the Antidepressant Effect of Cyperi Rhizoma through Soothing the Liver.Evidence-based complementary and alternative medicine : eCAM · 2021Article
- The CAGE-MiR-181b-5p-S1PR1 Axis Regulates Anticancer Drug Resistance and Autophagy in Gastric Cancer Cells.Frontiers in cell and developmental biology · 2021Article
- Plasminogen activator inhibitor-1: a potential etiological role in livedoid vasculopathy.International wound journal · 2020Review
- PAI-1, the Plasminogen System, and Skeletal Muscle.International journal of molecular sciences · 2020Review
- SGLT2 Inhibitor Empagliflozin and DPP4 Inhibitor Linagliptin Reactivate Glomerular Autophagy inInternational journal of molecular sciences · 2020Article
- A critical role for plasminogen in inflammation.The Journal of experimental medicine · 2020Review
- Proximity-dependent biotinylation screening identifies NbHYPK as a novel interacting partner of ATG8 in plants.BMC plant biology · 2019Article
- Proteome profiling of triple negative breast cancer cells overexpressing NOD1 and NOD2 receptors unveils molecular signatures of malignant cell proliferation.BMC genomics · 2019Article
- Molecular biomarkers of Graves' ophthalmopathy.Experimental and molecular pathology · 2019Review
- TGF-β1/p53 signaling in renal fibrogenesis.Cellular signalling · 2018Review
- Histone deacetylase 2 (HDAC2) attenuates lipopolysaccharide (LPS)-induced inflammation by regulating PAI-1 expression.Journal of inflammation (London, England) · 2018Article
- N-Acetylcysteine Attenuates the Development of Renal Fibrosis in Transgenic Mice with Dilated Cardiomyopathy.Scientific reports · 2017Article
- Integration of Canonical and Noncanonical Pathways in TLR4 Signaling: Complex Regulation of the Wound Repair Program.Advances in wound care · 2017Review
- Reduced monocyte adhesion to aortae of diabetic plasminogen activator inhibitor-1 knockout mice.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2017Article
- Recent insight into potential acute respiratory distress syndrome.Saudi medical journal · 2017Review
- A current pharmacologic agent versus the promise of next generation therapeutics to ameliorate protein misfolding and/or aggregation diseases.Current opinion in chemical biology · 2016Review
- The Effect of Autophagy on Inflammation Cytokines in Renal Ischemia/Reperfusion Injury.Inflammation · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe mechanisms by which plasminogen activator inhibitor-1 (PAI-1) regulates inflammation, especially in acute respiratory distress syndrome (ARDS), are largely unknown.
objectiveTo assess the relationship between PAI-1 and autophagy in inflammatory reactions induced by LPS in rat NR8383 cells.
methodsELISA was used to assess the amounts of TNF-α, IL-1β, and PAI-1 in cell culture supernatants; TLR4, MyD88, PAI-1, LC3, Beclin1, and mTOR protein and mRNA levels were determined by western blot and quantitative RT-PCR, respectively; western blot was used to determine NF-κB protein levels. To further evaluate the role of PAI-1, the PAI-1 gene was downregulated and overexpressed using the siRNA transfection technology and the pCDH-PAI-1, respectively. Finally, the GFP Positive Expression Rate Method was used to determine the rate of GFP-LC3 positive NR8383 cells.
resultsIn LPS-induced NR8383 cells, TNF-α, IL-1β, and PAI-1 expression levels increased remarkably. Upon PAI-1 knockdown, TNF-α, IL-1β, PAI-1, TLR4, MyD88, NF-κB, LC3, and Beclin1 levels were decreased, while mTOR increased. Conversely, overexpression of PAI-1 resulted in increased amounts of TNF-α, IL-1β, PAI-1, TLR4, MyD88, NF-κB, LC3, and Beclin1. However, no significant change was observed in mTOR expression.
conclusionsIn NR8383 cells, PAI-1 contributes in the regulation of LPS-induced inflammation, likely by promoting autophagy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.