ArticleMicrobial biotechnology2015
Degradation of toluene by ortho cleavage enzymes in Burkholderia fungorum FLU100.
Article in Microbial biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 45 citations in OpenAlex.
- Comprehensive genomic analysis of a novelMicrobiology spectrum · 2026Article
- The potential of Paraburkholderia species to enhance crop growth.World journal of microbiology & biotechnology · 2025Review
- Isolation and Genomic Analysis of 3-Chlorobenzoate-Degrading Bacteria from Soil.Microorganisms · 2023Article
- Genome sequence analysis of Malayan pangolin (PeerJ · 2023Article
- Isolation and Characterization a Novel Catabolic Gene Cluster Involved in Chlorobenzene Degradation in HaloalkaliphilicBiology · 2022Article
- Effect of metabolic uncouplers on the performance of toluene-degrading biotrickling filter.Environmental science and pollution research international · 2021Article
- Biodegradation of Chloroxylenol byInternational journal of molecular sciences · 2021Article
- Combination of highly efficient microflora to degrade paint spray exhaust gas.Scientific reports · 2020Article
- The Polycyclic Aromatic Hydrocarbon (PAH) degradation activities and genome analysis of a novel strainPeerJ · 2020Article
- Genome analysis of deep sea piezotolerant Nesiotobacter exalbescens COD22 and toluene degradation studies under high pressure condition.Scientific reports · 2019Article
- The Biodiversity Changes in the Microbial Population of Soils Contaminated with Crude Oil.Current microbiology · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia fungorum FLU100 simultaneously oxidized any mixture of toluene, benzene and mono-halogen benzenes to (3-substituted) catechols with a selectivity of nearly 100%. Further metabolism occurred via enzymes of ortho cleavage pathways with complete mineralization. During the transformation of 3-methylcatechol, 4-carboxymethyl-2-methylbut-2-en-4-olide (2-methyl-2-enelactone, 2-ML) accumulated transiently, being further mineralized only after a lag phase of 2 h in case of cells pre-grown on benzene or mono-halogen benzenes. No lag phase, however, occurred after growth on toluene. Cultures inhibited by chloramphenicol after growth on benzene or mono-halogen benzenes were unable to metabolize 2-ML supplied externally, even after prolonged incubation. A control culture grown with toluene did not show any lag phase and used 2-ML as a substrate. This means that 2-ML is an intermediate of toluene degradation and converted by specific enzymes. The conversion of 4-methylcatechol as a very minor by-product of toluene degradation in strain FLU100 resulted in the accumulation of 4-carboxymethyl-4-methylbut-2-en-4-olide (4-methyl-2-enelactone, 4-ML) as a dead-end product, excluding its nature as a possible intermediate. Thus, 3-methylcyclohexa-3,5-diene-1,2-diol, 3-methylcatechol, 2-methyl muconate and 2-ML were identified as central intermediates of productive ortho cleavage pathways for toluene metabolism in B. fungorum FLU100.
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