ArticleNature communications2014
Quantitative super-resolution imaging of Bruchpilot distinguishes active zone states.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 132 papers.
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Who cites it
132 citing papers in PubMed.
- Article
- Disruption of a selective vesicle pool upon retrograde amnesia dissociates memory at presynaptic terminals.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Active Zone: Linking Resolution Levels of Microscopic Modalities.Advances in neurobiology · 2026Review
- Octopamine signaling regulates the intracellular pattern of the presynaptic active zone scaffold within Drosophila mushroom body neurons.PLoS biology · 2025Article
- Deficiency in transmitter release triggers homeostatic transcriptional changes that increase presynaptic excitability.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Building and modifying diverse synaptic properties: Insights from Drosophila.Current opinion in neurobiology · 2025Review
- Fluorescent labeling strategies for molecular bioimaging.Biophysical reports · 2025Review
- Blobby is a synaptic active zone assembly protein required for memory in Drosophila.Nature communications · 2025Article
- Type-specific molecular signaling architectures and synaptic plasticity ofFrontiers in cellular neuroscience · 2025Article
- Cluster parameter-based DBSCAN maps for image characterization.Computational and structural biotechnology journal · 2025Article
- Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassium acrylate-based expansion microscopy.Nature communications · 2024Article
- FMR1 genetically interacts with DISC1 to regulate glutamatergic synaptogenesis.Schizophrenia (Heidelberg, Germany) · 2024Article
- CaeLife · 2024Article
- Article
- CabioRxiv : the preprint server for biology · 2024Article
- Skewing information flow through pre- and postsynaptic plasticity in the mushroom bodies ofLearning & memory (Cold Spring Harbor, N.Y.) · 2024Review
- Versatile Endogenous Editing of GluRIIA inCells · 2024Article
- Article
- Interplay between stochastic enzyme activity and microtubule stability drives detyrosination enrichment on microtubule subsets.Current biology : CB · 2023Article
- Nanoscaled RIM clustering at presynaptic active zones revealed by endogenous tagging.Life science alliance · 2023Article
72 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors.
Funding
Abstract
The precise molecular architecture of synaptic active zones (AZs) gives rise to different structural and functional AZ states that fundamentally shape chemical neurotransmission. However, elucidating the nanoscopic protein arrangement at AZs is impeded by the diffraction-limited resolution of conventional light microscopy. Here we introduce new approaches to quantify endogenous protein organization at single-molecule resolution in situ with super-resolution imaging by direct stochastic optical reconstruction microscopy (dSTORM). Focusing on the Drosophila neuromuscular junction (NMJ), we find that the AZ cytomatrix (CAZ) is composed of units containing ~137 Bruchpilot (Brp) proteins, three quarters of which are organized into about 15 heptameric clusters. We test for a quantitative relationship between CAZ ultrastructure and neurotransmitter release properties by engaging Drosophila mutants and electrophysiology. Our results indicate that the precise nanoscopic organization of Brp distinguishes different physiological AZ states and link functional diversification to a heretofore unrecognized neuronal gradient of the CAZ ultrastructure.
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