ArticleNephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association2014
Proteomic analysis of formalin-fixed paraffin-embedded glomeruli suggests depletion of glomerular filtration barrier proteins in two-kidney, one-clip hypertensive rats.
Article in Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 18 citations in OpenAlex.
- Comparative proteomic analysis of children FSGS FFPE tissues.BMC pediatrics · 2022Article
- Protein Extraction From FFPE Kidney Tissue Samples: A Review of the Literature and Characterization of Techniques.Frontiers in medicine · 2021Article
- Proteomics of human glomerulonephritis by laser microdissection and liquid chromatography-tandem mass spectrometry.Nephrology (Carlton, Vic.) · 2020Article
- Characterization of glomerular extracellular matrix in IgA nephropathy by proteomic analysis of laser-captured microdissected glomeruli.BMC nephrology · 2019Article
- Effects of Pravastatin in Adriamycin-Induced Nephropathy in Rats.Iranian journal of pharmaceutical research : IJPR · 2018Article
- Glomerular abundance of complement proteins characterized by proteomic analysis of laser-captured microdissected glomeruli associates with progressive disease in IgA nephropathy.Clinical proteomics · 2017Article
- Renal disease pathophysiology and treatment: contributions from the rat.Disease models & mechanisms · 2016Review
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9 authors at 2 institutions in 1 country.
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Abstract
backgroundIt is well known that hypertension may cause glomerular damage, but the molecular mechanisms involved are still incompletely understood.
methodsIn the present study, we used formalin-fixed paraffin-embedded (FFPE) tissue to investigate changes in the glomerular proteome in the non-clipped kidney of two-kidney one-clip (2K1C) hypertensive rats, with special emphasis on the glomerular filtration barrier. 2K1C hypertension was induced in 6-week-old Wistar Hannover rats (n = 6) that were sacrificed 23 weeks later and compared with age-matched sham-operated controls (n = 6). Tissue was stored in FFPE tissue blocks and later prepared on tissue slides for laser microdissection. Glomeruli without severe morphological damage were isolated, and the proteomes were analysed using liquid chromatography-tandem mass spectrometry.
results2K1C glomeruli showed reduced abundance of proteins important for slit diaphragm complex, such as nephrin, podocin and neph1. The podocyte foot process had a pattern of reduced abundance of transmembrane proteins but unchanged abundances of the podocyte cytoskeletal proteins synaptopodin and α-actinin-4. Lower abundance of important glomerular basement membrane proteins was seen. Possible glomerular markers of damage with increased abundance in 2K1C were transgelin, desmin and acyl-coenzyme A thioesterase 1.
conclusionsMicrodissection and tandem mass spectrometry could be used to investigate the proteome of isolated glomeruli from FFPE tissue. Glomerular filtration barrier proteins had reduced abundance in the non-clipped kidney of 2K1C hypertensive rats.
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