ArticleNature chemical biology2014
Discovery of a new ATP-binding motif involved in peptidic azoline biosynthesis.
Article in Nature chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
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- ThiF-Like Enzyme Chemistry in Primary and Secondary Metabolism.Chembiochem : a European journal of chemical biology · 2025Review
- Extending the Peptide/Protein Interaction Paradigm to a Protein/Protein Engagement Model in RiPP Biosynthesis.ACS chemical biology · 2025Article
- Assessing Substrate Scope of the Cyclodehydratase LynD by mRNA Display-Enabled Machine Learning Models.Biochemistry · 2025Article
- Biosynthesis of peptide-nucleobase hybrids in ribosomal peptides.Nature chemical biology · 2025Article
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- Uncovering the diversity and distribution of biosynthetic gene clusters of prochlorosins and other putative RiPPs in marineMicrobiology spectrum · 2024Article
- Bioinformatic mining for RiPP biosynthetic gene clusters in Bacteroidales reveals possible new subfamily architectures and novel natural products.Frontiers in microbiology · 2023Article
- Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets.Synthetic and systems biotechnology · 2022Article
- Review
- Control of Nucleophile Chemoselectivity in Cyanobactin YcaO Heterocyclases PatD and TruD.ACS chemical biology · 2022Article
- New Role for Radical SAM Enzymes in the Biosynthesis of Thio(seleno)oxazole RiPP Natural Products.Biochemistry · 2021Article
- Discovery and characterisation of an amidine-containing ribosomally-synthesised peptide that is widely distributed in nature.Chemical science · 2021Article
- Functional elucidation of TfuA in peptide backbone thioamidation.Nature chemical biology · 2021Article
- Global Genome Mining Reveals the Distribution of Diverse Thioamidated RiPP Biosynthesis Gene Clusters.Frontiers in microbiology · 2021Article
- New developments in RiPP discovery, enzymology and engineering.Natural product reports · 2021Review
- Biosynthesis Gene Cluster and Oxazole Ring Formation Enzyme for Inthomycins inApplied and environmental microbiology · 2020Article
- Minimal lactazole scaffold for in vitro thiopeptide bioengineering.Nature communications · 2020Article
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Authors and funding
6 authors.
Funding
Abstract
Despite intensive research, the cyclodehydratase responsible for azoline biogenesis in thiazole/oxazole-modified microcin (TOMM) natural products remains enigmatic. The collaboration of two proteins, C and D, is required for cyclodehydration. The C protein is homologous to E1 ubiquitin-activating enzymes, whereas the D protein is within the YcaO superfamily. Recent studies have demonstrated that TOMM YcaOs phosphorylate amide carbonyl oxygens to facilitate azoline formation. Here we report the X-ray crystal structure of an uncharacterized YcaO from Escherichia coli (Ec-YcaO). Ec-YcaO harbors an unprecedented fold and ATP-binding motif. This motif is conserved among TOMM YcaOs and is required for cyclodehydration. Furthermore, we demonstrate that the C protein regulates substrate binding and catalysis and that the proline-rich C terminus of the D protein is involved in C protein recognition and catalysis. This study identifies the YcaO active site and paves the way for the characterization of the numerous YcaO domains not associated with TOMM biosynthesis.
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