ArticleThe Journal of biological chemistry2014
The crystal structure of the plant small GTPase OsRac1 reveals its mode of binding to NADPH oxidase.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 44 citations in OpenAlex.
- Nucleotide-dependent switching and RIPb effector recognition of the barley susceptibility factor RACB.Communications biology · 2026Article
- Interplay Between ROS and Hormones in Plant Defense Against Pathogens.Plants (Basel, Switzerland) · 2025Review
- Identification of NADPH Oxidase Genes Crucial for Rice Multiple Disease Resistance and Yield Traits.Rice (New York, N.Y.) · 2024Article
- Receptor for Activated C Kinase1B (OsRACK1B) Impairs Fertility in Rice through NADPH-Dependent HInternational journal of molecular sciences · 2022Article
- Rice functional genomics: decades' efforts and roads ahead.Science China. Life sciences · 2022Review
- Identification of a defense response gene involved in signaling pathways against PVA and PVY in potato.GM crops & food · 2021Article
- Genetic and Molecular Factors Determining Grain Weight in Rice.Frontiers in plant science · 2021Review
- Review
- Mutations of two FERONIA-like receptor genes enhance rice blast resistance without growth penalty.Journal of experimental botany · 2020Article
- Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa).BMC plant biology · 2020Article
- Every Coin Has Two Sides: Reactive Oxygen Species during Rice⁻International journal of molecular sciences · 2019Review
- ROP GTPases Structure-Function and Signaling Pathways.Plant physiology · 2018Review
- Crosstalk of Signaling Mechanisms Involved in Host Defense and Symbiosis Against Microorganisms in Rice.Current genomics · 2016Article
- Plasma Membrane Microdomains Are Essential for Rac1-RbohB/H-Mediated Immunity in Rice.The Plant cell · 2016Article
- Focusing on the focus: what else beyond the master switches for polar cell growth?Molecular plant · 2015Review
- New insights into the dimerization of small GTPase Rac/ROP guanine nucleotide exchange factors in rice.Plant signaling & behavior · 2015Article
- Rho family GTPase-dependent immunity in plants and animals.Frontiers in plant science · 2014Review
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rac/Rop proteins are Rho-type small GTPases that act as molecular switches in plants. Recent studies have identified these proteins as key components in many major plant signaling pathways, such as innate immunity, pollen tube growth, and root hair formation. In rice, the Rac/Rop protein OsRac1 plays an important role in regulating the production of reactive oxygen species (ROS) by the NADPH oxidase OsRbohB during innate immunity. However, the molecular mechanism by which OsRac1 regulates OsRbohB remains unknown. Here, we report the crystal structure of OsRac1 complexed with the non-hydrolyzable GTP analog guanosine 5'-(β,γ-imido)triphosphate at 1.9 Å resolution; this represents the first active-form structure of a plant small GTPase. To elucidate the ROS production in rice cells, structural information was used to design OsRac1 mutants that displayed reduced binding to OsRbohB. Only mutations in the OsRac1 Switch I region showed attenuated interactions with OsRbohB in vitro. In particular, Tyr(39) and Asp(45) substitutions suppressed ROS production in rice cells, indicating that these residues are critical for interaction with and activation of OsRbohB. Structural comparison of active-form OsRac1 with AtRop9 in its GDP-bound inactive form showed a large conformational difference in the vicinity of these residues. Our results provide new insights into the molecular mechanism of the immune response through OsRac1 and the various cellular responses associated with plant Rac/Rop proteins.
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