ArticleG3 (Bethesda, Md.)2014
Performance of the Cas9 nickase system in Drosophila melanogaster.
Article in G3 (Bethesda, Md.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Tuning mitotic recombination with patterned DNA nicks for precision mosaic analysis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management.Plants (Basel, Switzerland) · 2023Review
- Article
- A nickase Cas9 gene-drive system promotes super-Mendelian inheritance in Drosophila.Cell reports · 2022Article
- State-of-the-art CRISPR for in vivo and cell-based studies in Drosophila.Trends in genetics : TIG · 2022Review
- Transposable element landscapes in aging Drosophila.PLoS genetics · 2022Article
- Article
- Enhanced Efficiency of flySAM by Optimization of sgRNA Parameters inG3 (Bethesda, Md.) · 2020Article
- Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species.Nucleic acids research · 2020Article
- CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool.Antibiotics (Basel, Switzerland) · 2019Review
- Review
- Progress and Prospects of CRISPR/Cas Systems in Insects and Other Arthropods.Frontiers in physiology · 2017Review
- Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Coconversion.G3 (Bethesda, Md.) · 2016Article
- Advances and perspectives in the application of CRISPR/Cas9 in insects.Dong wu xue yan jiu = Zoological research · 2016Review
- DNA-free genome editing methods for targeted crop improvement.Plant cell reports · 2016Review
- Gene targeting, genome editing: from Dolly to editors.Transgenic research · 2016Review
- A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation.Plant physiology · 2015Article
- Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila.Cell reports · 2014Article
- A versatile two-step CRISPR- and RMCE-based strategy for efficient genome engineering in Drosophila.G3 (Bethesda, Md.) · 2014Article
- Genome engineering: Drosophila melanogaster and beyond.Wiley interdisciplinary reviews. Developmental biologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
Recent studies of the Cas9/sgRNA system in Drosophila melanogaster genome editing have opened new opportunities to generate site-specific mutant collections in a high-throughput manner. However, off-target effects of the system are still a major concern when analyzing mutant phenotypes. Mutations converting Cas9 to a DNA nickase have great potential for reducing off-target effects in vitro. Here, we demonstrated that injection of two plasmids encoding neighboring offset sgRNAs into transgenic Cas9(D10A) nickase flies efficiently produces heritable indel mutants. We then determined the effective distance between the two sgRNA targets and their orientations that affected the ability of the sgRNA pairs to generate mutations when expressed in the transgenic nickase flies. Interestingly, Cas9 nickase greatly reduces the ability to generate mutants with one sgRNA, suggesting that the application of Cas9 nickase and sgRNA pairs can almost avoid off-target effects when generating indel mutants. Finally, a defined piwi mutant allele is generated with this system through homology-directed repair. However, Cas9(D10A) is not as effective as Cas9 in replacing the entire coding sequence of piwi with two sgRNAs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.