ArticleProceedings of the National Academy of Sciences of the United States of America2014
Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 71 citations in OpenAlex.
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- A combined PIE-FRET and FCS assay to monitor RNA dynamics and cleavage by SARS-CoV-2 Nsp15.Research square · 2025Article
- Genome-wide profiling of tRNA modifications by Induro-tRNAseq reveals coordinated changes.Nature communications · 2025Article
- Suppressor tRNA in gene therapy.Science China. Life sciences · 2024Review
- Engineering tRNAs for the Ribosomal Translation of Non-proteinogenic Monomers.Chemical reviews · 2024Review
- Anticodon stem-loop tRNA modifications influence codon decoding and frame maintenance during translation.Seminars in cell & developmental biology · 2024Review
- Tuning tRNAs for improved translation.Frontiers in genetics · 2024Review
- tRNA methylation resolves codon usage bias at the limit of cell viability.Cell reports · 2022Article
- mRNA and tRNA modification states influence ribosome speed and frame maintenance during poly(lysine) peptide synthesis.The Journal of biological chemistry · 2022Article
- Structural and functional characterization of TrmM in mProtein science : a publication of the Protein Society · 2022Article
- Genome Expansion by tRNA +1 Frameshifting at Quadruplet Codons.Journal of molecular biology · 2022Review
- Variance in translational fidelity of different bacterial species is affected by pseudouridines in the tRNA anticodon stem-loop.RNA biology · 2022Article
- Thinking Outside the Frame: Impacting Genomes Capacity by Programmed Ribosomal Frameshifting.Frontiers in molecular biosciences · 2022Review
- Twice exploration of tRNA +1 frameshifting in an elongation cycle of protein synthesis.Nucleic acids research · 2021Article
- The macronuclear genome of the Antarctic psychrophilic marine ciliate Euplotes focardii reveals new insights on molecular cold adaptation.Scientific reports · 2021Article
- Loss ofeLife · 2021Article
- Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation.Nature communications · 2021Article
- High-resolution quantitative profiling of tRNA abundance and modification status in eukaryotes by mim-tRNAseq.Molecular cell · 2021Article
- Transfer RNAs: diversity in form and function.RNA biology · 2021Review
- Insights into genome recoding from the mechanism of a classic +1-frameshifting tRNA.Nature communications · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Maintenance of the correct reading frame on the ribosome is essential for accurate protein synthesis. Here, we report structures of the 70S ribosome bound to frameshift suppressor tRNA(SufA6) and N1-methylguanosine at position 37 (m(1)G37) modification-deficient anticodon stem loop(Pro), both of which cause the ribosome to decode 4 rather than 3 nucleotides, resulting in a +1 reading frame. Our results reveal that decoding at +1 suppressible codons causes suppressor tRNA(SufA6) to undergo a rearrangement of its 5' stem that destabilizes U32, thereby disrupting the conserved U32-A38 base pair. Unexpectedly, the removal of the m(1)G37 modification of tRNA(Pro) also disrupts U32-A38 pairing in a structurally analogous manner. The lack of U32-A38 pairing provides a structural correlation between the transition from canonical translation and a +1 reading of the mRNA. Our structures clarify the molecular mechanism behind suppressor tRNA-induced +1 frameshifting and advance our understanding of the role played by the ribosome in maintaining the correct translational reading frame.
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