ArticleTissue engineering. Part C, Methods2015
Application and assessment of optical clearing methods for imaging of tissue-engineered neural stem cell spheres.
Article in Tissue engineering. Part C, Methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Experimental workflow to evaluate nanoparticles combined with photon and carbon-ion medical irradiations in spheroids.Discover nano · 2026Article
- Advanced tissue technologies of blood-brain barrier organoids as high throughput toxicity readouts in drug development.Heliyon · 2025Article
- Rigor and reproducibility in human brain organoid research: Where we are and where we need to go.Stem cell reports · 2024Review
- Deep learning-based adaptive optics for light sheet fluorescence microscopy.Biomedical optics express · 2023Article
- On-chip clearing for live imaging of 3D cell cultures.Biomedical optics express · 2023Article
- Article
- Quantitative analysis of illumination and detection corrections in adaptive light sheet fluorescence microscopy.Biomedical optics express · 2022Article
- Distinct cholangiocarcinoma cell migration in 2D monolayer and 3D spheroid culture based on galectin-3 expression and localization.Frontiers in oncology · 2022Article
- Recent progresses in novelFrontiers in bioengineering and biotechnology · 2022Review
- Single cell organization and cell cycle characterization of DNA stained multicellular tumor spheroids.Scientific reports · 2021Article
- An integrated framework for quantifying immune-tumour interactions in a 3D co-culture model.Communications biology · 2021Article
- Factors to consider when interrogating 3D culture models with plate readers or automated microscopes.In vitro cellular & developmental biology. Animal · 2021Review
- New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution.Viruses · 2021Article
- Perspective: Extending the Utility of Three-Dimensional Organoids by Tissue Clearing Technologies.Frontiers in cell and developmental biology · 2021Article
- Three-Dimensional Imaging in Stem Cell-Based Researches.Frontiers in veterinary science · 2021Review
- NovelFrontiers in cellular neuroscience · 2021Review
- Influence ofInternational journal of molecular sciences · 2020Article
- Rapid Clearing for High Resolution 3D Imaging of Ex Vivo Pancreatic Cancer Spheroids.International journal of molecular sciences · 2020Article
- Mapping the structure and biological functions within mesenchymal bodies using microfluidics.Science advances · 2020Article
- Spatial heterogeneity of nanomedicine investigated by multiscale imaging of the drug, the nanoparticle and the tumour environment.Theranostics · 2020Review
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2 authors.
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Abstract
Three-dimensional (3D) cell culture is an important tool that facilitates biological discoveries by bridging the divide between standard two-dimensional cell culture and the complex, high-cell-density in vivo environment. Typically, the internal structures of 3D tissue-engineered samples are visualized through an involved process of physical sectioning, immunostaining, imaging, and computational reconstruction. However, recent progress in tissue-clearing methods has improved optical-imaging-depth capabilities in whole embryos and brains by reducing tissue opacity and light scattering, thus decreasing the need for physical sectioning. In this study, we assessed the application of the recently published clearing techniques Clear(T2), Scale, and SeeDB to tissue-engineered neural spheres. We found that scaffold-free self-assembled adult hippocampal neural stem cell spheres of 100-μm diameter could be optically cleared and imaged using either Clear(T2) or Scale, while SeeDB only marginally improved imaging depth. The Clear(T2) protocol maintained sphere size, while Scale led to sample expansion, and SeeDB led to sample shrinkage. Additionally, using Clear(T2) we cleared and successfully imaged spheres of C6 glioma cells and spheres of primary cortical neurons. We conclude that Clear(T2) is the most effective protocol of those tested at clearing neural spheres of various cell types and could be applied to better understand neural cell interactions in 3D tissue-engineered samples.
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