ArticleDevelopmental biology2014
In vivo analysis of hyaloid vasculature morphogenesis in zebrafish: A role for the lens in maturation and maintenance of the hyaloid.
Article in Developmental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Zebrafish col4a1 loss-of-function models mirror key neurovascular and ocular features of COL4A1/A2 syndrome and enable human variants assessment in vivo.Matrix biology : journal of the International Society for Matrix Biology · 2026Article
- Adaptive Optical Two-Photon Fluorescence Microscopy Probes Cellular Organization of Ocular Lenses In Vivo.Investigative ophthalmology & visual science · 2023Article
- Identification of HSPA8 as an interacting partner of MAB21L2 and an important factor in eye development.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Article
- Adaptive optical two-photon fluorescence microscopy probes cellular organization of ocular lenses in vivo.bioRxiv : the preprint server for biology · 2023Article
- Systemic treatment with cigarette smoke extract affects zebrafish visual behaviour, intraocular vasculature morphology and outer segment phagocytosis.Open research Europe · 2023Article
- Heterogeneities of zebrafish vasculature development studied by a high throughput light-sheet flow imaging system.Biomedical optics express · 2022Article
- Zebrafish models of alx-linked frontonasal dysplasia reveal a role for Alx1 and Alx3 in the anterior segment and vasculature of the developing eye.Biology open · 2022Article
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- A versatile, automated and high-throughput drug screening platform for zebrafish embryos.Biology open · 2021Article
- Genetic disruption of zebrafish mab21l1 reveals a conserved role in eye development and affected pathways.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- Build me up optic cup: Intrinsic and extrinsic mechanisms of vertebrate eye morphogenesis.Developmental biology · 2021Review
- Zebrafish Model in Ophthalmology to Study Disease Mechanism and Drug Discovery.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Identification of missense MAB21L1 variants in microphthalmia and aniridia.Human mutation · 2021Article
- Semaphorin 3fa Controls Ocular Vascularization From the Embryo Through to the Adult.Investigative ophthalmology & visual science · 2021Article
- Selective Requirements for Vascular Endothelial Cells and Circulating Factors in the Regulation of Retinal Neurogenesis.Frontiers in cell and developmental biology · 2021Article
- Disruption of foxc1 genes in zebrafish results in dosage-dependent phenotypes overlapping Axenfeld-Rieger syndrome.Human molecular genetics · 2020Article
- Hyaloid vasculature and mmp2 activity play a role during optic fissure fusion in zebrafish.Scientific reports · 2020Article
- Microglia in the developing retina couple phagocytosis with the progression of apoptosis via P2RY12 signaling.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- Zebrafish mab21l2 mutants possess severe defects in optic cup morphogenesis, lens and cornea development.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Article
- Transcriptome profiling of zebrafish optic fissure fusion.Scientific reports · 2019Article
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4 authors.
Funding
Abstract
Two vascular networks nourish the embryonic eye as it develops - the hyaloid vasculature, located at the anterior of the eye between the retina and lens, and the choroidal vasculature, located at the posterior of the eye, surrounding the optic cup. Little is known about hyaloid development and morphogenesis, however. To begin to identify the morphogenetic underpinnings of hyaloid formation, we utilized in vivo time-lapse confocal imaging to characterize morphogenesis of the zebrafish hyaloid through 5 days post fertilization (dpf). Our data segregate hyaloid formation into three distinct morphogenetic stages: Stage I: arrival of hyaloid cells at the lens and formation of the hyaloid loop; Stage II: formation of a branched hyaloid network; Stage III: refinement of the hyaloid network. Utilizing fixed and dissected tissues, distinct Stage II and Stage III aspects of hyaloid formation were quantified over time. Combining in vivo imaging with microangiography, we demonstrate that the hyaloid system becomes fully enclosed by 5dpf. To begin to identify the molecular and cellular mechanisms underlying hyaloid morphogenesis, we identified a recessive mutation in the mab21l2 gene, and in a subset of mab21l2 mutants the lens does not form. Utilizing these "lens-less" mutants, we determined whether the lens was required for hyaloid morphogenesis. Our data demonstrate that the lens is not required for Stage I of hyaloid formation; however, Stages II and III of hyaloid formation are disrupted in the absence of a lens, supporting a role for the lens in hyaloid maturation and maintenance. Taken together, this study provides a foundation on which the cellular, molecular and embryologic mechanisms underlying hyaloid morphogenesis can be elucidated.
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