ArticlePloS one2014
Do cells contribute to tendon and ligament biomechanics?
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Trial
- Crosado embalming related alterations in the morpho-mechanics of collagen rich tissues.Scientific reports · 2025Article
- On a potential morpho-mechanical link between the gluteus maximus muscle and pelvic floor tissues.Scientific reports · 2023Article
- Biomechanical characterization of human temporal muscle fascia in uniaxial tensile tests for graft purposes in duraplasty.Scientific reports · 2021Article
- Surface coating and speckling of the human iliotibial tract does not affect its load-deformation properties.Scientific reports · 2020Article
- Load-deformation characteristics of acellular human scalp: assessing tissue grafts from a material testing perspective.Scientific reports · 2020Article
- What is Considered a Variation of Biomechanical Parameters in Tensile Tests of Collagen-Rich Human Soft Tissues? - Critical Considerations Using the Human Cranial Dura Mater as a Representative Morpho-Mechanic Model.Medicina (Kaunas, Lithuania) · 2020Article
- Article
- An ossifying bridge - on the structural continuity between the Achilles tendon and the plantar fascia.Scientific reports · 2020Article
- Strain Assessment of Deep Fascia of the Thigh During Leg Movement: AnFrontiers in bioengineering and biotechnology · 2020Article
- Mechanical Properties of Human Dura Mater in Tension - An Analysis at an Age Range of 2 to 94 Years.Scientific reports · 2019Article
- Water-content related alterations in macro and micro scale tendon biomechanics.Scientific reports · 2019Article
- An Investigation on the Correlation between the Mechanical Properties of Human Skull Bone, Its Geometry, Microarchitectural Properties, and Water Content.Journal of healthcare engineering · 2019Article
- Cryopreservation of tendon tissue using dimethyl sulfoxide combines conserved cell vitality with maintained biomechanical features.PloS one · 2019Article
- Utilization of 3D printing technology to facilitate and standardize soft tissue testing.Scientific reports · 2018Article
- Load and failure behavior of human muscle samples in the context of proximal femur replacement.BMC musculoskeletal disorders · 2016Article
- Article
- Engineering Tendon: Scaffolds, Bioreactors, and Models of Regeneration.Stem cells international · 2016Review
- Actin cytoskeleton contributes to the elastic modulus of embryonic tendon during early development.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2015Article
- Fresh versus frozen engineered bone-ligament-bone grafts for sheep anterior cruciate ligament repair.Tissue engineering. Part C, Methods · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionAcellular scaffolds are increasingly used for the surgical repair of tendon injury and ligament tears. Despite this increased use, very little data exist directly comparing acellular scaffolds and their native counterparts. Such a comparison would help establish the effectiveness of the acellularization procedure of human tissues. Furthermore, such a comparison would help estimate the influence of cells in ligament and tendon stability and give insight into the effects of acellularization on collagen. MATERIAL AND
methodsEighteen human iliotibial tract samples were obtained from nine body donors. Nine samples were acellularized with sodium dodecyl sulphate (SDS), while nine counterparts from the same donors remained in the native condition. The ends of all samples were plastinated to minimize material slippage. Their water content was adjusted to 69%, using the osmotic stress technique to exclude water content-related alterations of the mechanical properties. Uniaxial tensile testing was performed to obtain the elastic modulus, ultimate stress and maximum strain. The effectiveness of the acellularization procedure was histologically verified by means of a DNA assay.
resultsThe histology samples showed a complete removal of the cells, an extensive, yet incomplete removal of the DNA content and alterations to the extracellular collagen. Tensile properties of the tract samples such as elastic modulus and ultimate stress were unaffected by acellularization with the exception of maximum strain. DISCUSSION: The data indicate that cells influence the mechanical properties of ligaments and tendons in vitro to a negligible extent. Moreover, acellularization with SDS alters material properties to a minor extent, indicating that this method provides a biomechanical match in ligament and tendon reconstruction. However, the given protocol insufficiently removes DNA. This may increase the potential for transplant rejection when acellular tract scaffolds are used in soft tissue repair. Further research will help optimize the SDS-protocol for clinical application.
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