ArticleAutophagy2014
Correlative light and electron microscopy imaging of autophagy in a zebrafish infection model.
Article in Autophagy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 57 citations in OpenAlex.
- PIKfyve mediates the maturation of mycobacteria-containing vesicles.Frontiers in cellular and infection microbiology · 2026Article
- Evolving strategies of intracellular HypervirulentVirulence · 2025Article
- Targeting Autophagy as a Strategy for Developing New Host-Directed Therapeutics Against Nontuberculous Mycobacteria.Pathogens (Basel, Switzerland) · 2025Review
- Hyphal growth determines spatial organization and coexistence in a pathogenic polymicrobial community in a spatially structured environment.The ISME journal · 2025Article
- Autophagy in aquatic animals: mechanisms, implications, and future directions.Frontiers in immunology · 2025Review
- Using Zebrafish to Dissect the Interaction of Mycobacteria with the Autophagic Machinery in Macrophages.Biology · 2023Article
- Article
- Xenophagy receptors Optn and p62 and autophagy modulator Dram1 independently promote the zebrafish host defense againstFrontiers in cellular and infection microbiology · 2023Article
- A fresh look at mycobacterial pathogenicity with the zebrafish host model.Molecular microbiology · 2022Review
- Specificity of the innate immune responses to different classes of non-tuberculous mycobacteria.Frontiers in immunology · 2022Article
- Imaging Infection Across Scales of Size: From Whole Animals to Single Molecules.Annual review of microbiology · 2021Review
- Autophagy alleviates hypoxia-induced blood-brain barrier injury via regulation of CLDN5 (claudin 5).Autophagy · 2021Article
- The adapter protein Myd88 plays an important role in limiting mycobacterial growth in a zebrafish model for tuberculosis.Virchows Archiv : an international journal of pathology · 2021Article
- Zebrafish larvae as experimental model to expedite the search for new biomarkers and treatments for neonatal sepsis.Journal of clinical and translational science · 2021Review
- Autophagy and Host Defense in Nontuberculous Mycobacterial Infection.Frontiers in immunology · 2021Review
- A Human Osteocyte Cell Line Model for StudyingFrontiers in cellular and infection microbiology · 2021Article
- Article
- Review
- Early cell-autonomous accumulation of neutral lipids during infection promotes mycobacterial growth.PloS one · 2020Article
- Mycobacterium tuberculosis infection of host cells in space and time.FEMS microbiology reviews · 2019Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-resolution imaging of autophagy has been used intensively in cell culture studies, but so far it has been difficult to visualize this process in detail in whole animal models. In this study we present a versatile method for high-resolution imaging of microbial infection in zebrafish larvae by injecting pathogens into the tail fin. This allows visualization of autophagic compartments by light and electron microscopy, which makes it possible to correlate images acquired by the 2 techniques. Using this method we have studied the autophagy response against Mycobacterium marinum infection. We show that mycobacteria during the progress of infection are frequently associated with GFP-Lc3-positive vesicles, and that 2 types of GFP-Lc3-positive vesicles were observed. The majority of these vesicles were approximately 1 μm in size and in close vicinity of bacteria, and a smaller number of GFP-Lc3-positive vesicles was larger in size and were observed to contain bacteria. Quantitative data showed that these larger vesicles occurred significantly more in leukocytes than in other cell types, and that approximately 70% of these vesicles were positive for a lysosomal marker. Using electron microscopy, it was found that approximately 5% of intracellular bacteria were present in autophagic vacuoles and that the remaining intracellular bacteria were present in phagosomes, lysosomes, free inside the cytoplasm or occurred as large aggregates. Based on correlation of light and electron microscopy images, it was shown that GFP-Lc3-positive vesicles displayed autophagic morphology. This study provides a new approach for injection of pathogens into the tail fin, which allows combined light and electron microscopy imaging in vivo and opens new research directions for studying autophagy process related to infectious diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.