Evidence map›Paper›PMID 25126483›Full record

ArticleBioResearch open access2014

High resolution x-ray: a reliable approach for quantifying osteoporosis in a rodent model.

Omar Velasco, Aaron W James, Greg Asatrian, Mark Ajalat, Tyler Pritchard, Siyouneh Novshadian, Anu Murthy, Georgina Bayani, Xinli Zhang, Kang Ting and 1 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioResearch open access, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Mkx-Deficient Mice Exhibit Hedgehog Signaling-Dependent Ectopic Ossification in the Achilles Tendons.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Omar VelascoDental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, University of California , Los Angeles, California. ; Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, UCLA and Orthopaedic Hospital, University of California , Los Angeles, California. ; Department of Surgery, University of Maryland Medical Center , Baltimore, Maryland.
Aaron W JamesDental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, University of California , Los Angeles, California. ; Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, UCLA and Orthopaedic Hospital, University of California , Los Angeles, California. ; Department of Pathology and Laboratory Medicine, University of California , Los Angeles, California.
Greg AsatrianDental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, University of California , Los Angeles, California.
Mark AjalatDental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, University of California , Los Angeles, California. ; Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, UCLA and Orthopaedic Hospital, University of California , Los Angeles, California.
Tyler PritchardDental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, University of California , Los Angeles, California. ; Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, UCLA and Orthopaedic Hospital, University of California , Los Angeles, California.
Siyouneh NovshadianDental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, University of California , Los Angeles, California.
Anu MurthyDental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, University of California , Los Angeles, California.
Georgina BayaniDental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, University of California , Los Angeles, California. ; Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, UCLA and Orthopaedic Hospital, University of California , Los Angeles, California.
Xinli ZhangDental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, University of California , Los Angeles, California. ; Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, UCLA and Orthopaedic Hospital, University of California , Los Angeles, California.
Kang TingDental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, University of California , Los Angeles, California. ; Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, UCLA and Orthopaedic Hospital, University of California , Los Angeles, California.
Chia SooDepartment of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, UCLA and Orthopaedic Hospital, University of California , Los Angeles, California. ; Division of Plastic and Reconstructive Surgery, Department of Surgery, David Geffen School of Medicine, University of California , Los Angeles, California.
National Institute of Dental and Craniofacial Research · USUniversity of California, Los Angeles · USUniversity of Maryland, Baltimore · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Osteoporosis is the most common metabolic disease of bone, resulting in significant worldwide morbidity. Currently, there are insufficient imaging modalities available to evaluate osteoporotic bones in small animal models. Here, we demonstrate the feasibility of using high resolution X-ray imaging as a comparable measure of bone degeneration to dual-energy X-ray absorptiometry (DXA) in an osteoporosis rodent model. At week 0, animals underwent either an ovariectomy (OVX) or sham procedure (SHAM). DXA analysis was performed weekly to confirm and compare the bone degenerative changes induced by OVX. A comparison using high resolution X-ray imaging (Faxitron(®)) was then performed postmortem due to need of soft tissue removal. Two regions of interest (ROIs) were utilized: the distal third of the femur and the lumbar spine (L4/L5). It was observed that SHAM animals maintained a relatively constant bone mineral density (BMD), in comparison to OVX animals, whereby a significant decrease in BMD was appreciated. Post mortem X-ray scans were performed and converted to 8-bit color and quantified. A high level of agreement with DXA quantifications was observed with X-ray quantifications, and a significant correlation between the radiopacity, visualized by color distributions, and the DXA BMD values between animal groups was evident. Our study demonstrates the applicability of high resolution X-ray imaging both qualitatively and quantitatively as a reliable approach for quantifying osteoporosis in rodent osteoporotic models. With DXA being a highly user dependent modality, our technique is a unique secondary methodology to verify DXA findings and minimize inter-observer variability.

Indexed as

dual-energy X-ray absorptiometryDXAosteoporosisovariectomyX-ray

Identifiers

PMID25126483
PMCPMC4120930
OpenAlexW2066248188

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.