Evidence map›Paper›PMID 25126480›Full record

ArticleBioResearch open access2014

Directing human induced pluripotent stem cells into a neurosensory lineage for auditory neuron replacement.

Niliksha Gunewardene, Nicole Van Bergen, Duncan Crombie, Karina Needham, Mirella Dottori, Bryony A Nayagam

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioResearch open access, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 45 citations in OpenAlex.

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  6. Modern In Vitro Techniques for Modeling Hearing Loss.Bioengineering (Basel, Switzerland) · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Niliksha GunewardeneDepartment of Otolaryngology, University of Melbourne , East Melbourne, Victoria, Australia .
Nicole Van BergenCentre for Eye Research Australia, University of Melbourne , East Melbourne, Victoria, Australia .
Duncan CrombieCentre for Eye Research Australia, University of Melbourne , East Melbourne, Victoria, Australia .
Karina NeedhamDepartment of Otolaryngology, University of Melbourne , East Melbourne, Victoria, Australia .
Mirella DottoriCentre for Neural Engineering, University of Melbourne , Parkville, Victoria, Australia .
Bryony A NayagamCentre for Eye Research Australia, University of Melbourne , East Melbourne, Victoria, Australia . ; Department of Audiology and Speech Pathology, University of Melbourne , Parkville, Victoria, Australia . ; Bionics Institute, University of Melbourne , East Melbourne, Victoria, Australia .
The University of Melbourne · AUBionics Institute · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Emerging therapies for sensorineural hearing loss include replacing damaged auditory neurons (ANs) using stem cells. Ultimately, it is important that these replacement cells can be patient-matched to avoid immunorejection. As human induced pluripotent stem cells (hiPSCs) can be obtained directly from the patient, they offer an opportunity to generate patient-matched neurons for transplantation. Here, we used an established neural induction protocol to differentiate two hiPSC lines (iPS1 and iPS2) and one human embryonic stem cell line (hESC; H9) toward a neurosensory lineage in vitro. Immunocytochemistry and qRT-PCR were used to analyze the expression of key markers involved in AN development at defined time points of differentiation. The hiPSC- and hESC-derived neurosensory progenitors expressed the dorsal hindbrain marker (PAX7), otic placodal marker (PAX2), proneurosensory marker (SOX2), ganglion neuronal markers (NEUROD1, BRN3A, ISLET1, ßIII-tubulin, Neurofilament kDa 160), and sensory AN markers (GATA3 and VGLUT1) over the time course examined. The hiPSC- and hESC-derived neurosensory progenitors had the highest expression levels of the sensory neural markers at 35 days in vitro. Furthermore, the neurons generated from this assay were found to be electrically active. While all cell lines analyzed produced functional neurosensory-like progenitors, variabilities in the levels of marker expression were observed between hiPSC lines and within samples of the same cell line, when compared with the hESC controls. Overall, these findings indicate that this neural assay was capable of differentiating hiPSCs toward a neurosensory lineage but emphasize the need for improving the consistency in the differentiation of hiPSCs into the required lineages.

Indexed as

auditory neuroncochlear implantdeafnessdifferentiationhiPSCstem cell

Identifiers

PMID25126480
PMCPMC4120935
OpenAlexW5426716

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.