Evidence map›Paper›PMID 25126177›Full record

ArticleInternational journal of clinical and experimental medicine2014

Human umbilical cord mesenchymal stromal cells suppress MHC class II expression on rat vascular endothelium and prolong survival time of cardiac allograft.

Ying Qiu, Mark M Yun, Xia Han, Ruidong Zhao, Erxia Zhou, Sheng Yun

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In one paragraph

Article in International journal of clinical and experimental medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Review
  6. The application of umbilical cord-derived MSCs in cardiovascular diseases.Journal of cellular and molecular medicine · 2021
    Review
  7. Review
  8. Article
  9. Superparamagnetic iron oxide promotes osteogenic differentiation of rat adipose-derived stem cells.International journal of clinical and experimental medicine · 2015
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ying QiuStem Cell Centre, The First Teaching Hospital, Inner Mongolia Medical University Huhhot, 010050, China ; Department of Clinical Sciences, King's College London SE5 9NU, UK.
Mark M YunUCL Medical School, University College London Gower Street, London, WC1E 6BT, UK ; Present address: Heart of England, NHS FT Bordesley Green, East Birmingham, Bq 5ST, UK.
Xia HanStem Cell Centre, The First Teaching Hospital, Inner Mongolia Medical University Huhhot, 010050, China.
Ruidong ZhaoStem Cell Centre, The First Teaching Hospital, Inner Mongolia Medical University Huhhot, 010050, China.
Erxia ZhouStem Cell Centre, The First Teaching Hospital, Inner Mongolia Medical University Huhhot, 010050, China.
Sheng YunStem Cell Centre, The First Teaching Hospital, Inner Mongolia Medical University Huhhot, 010050, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHuman umbilical cord mesenchymal stromal cells (UC-MSCs) have low immunogenicity and immune regulation. To investigate immunomodulatory effects of human UC-MSCs on MHC class II expression and allograft, we transplanted heart of transgenic rats with MHC class II expression on vascular endothelium.

methodsUC-MSCs were obtained from human umbilical cords and confirmed with flow cytometry analysis. Transgenic rat line was established using the construct of human MHC class II transactivator gene (CIITA) under mouse ICAM-2 promoter control. The induced MHC class II expression on transgenic rat vascular endothelial cells (VECs) was assessed with immunohistological staining. And the survival time of cardiac allograft was compared between the recipients with and without UC-MSC transfusion.

resultsFlow cytometry confirmed that the human UC-MSCs were positive for CD29, CD44, CD73, CD90, CD105, CD271, and negative for CD34 and HLA-DR. Repeated infusion of human UC-MSCs reduced MHC class II expression on vascular endothelia of transplanted hearts, and increased survival time of allograft. The UC-MSCs increased regulatory cytokines IL10, transforming growth factor (TGF)-β1 and suppressed proinflammatory cytokines IL2 and IFN-γ in vivo. The UC-MSC culture supernatant had similar effects on cytokine expression, and decreased lymphocyte proliferation in vitro.

conclusionsRepeated transfusion of the human UC-MSCs reduced MHC class II expression on vascular endothelia and prolonged the survival time of rat cardiac allograft.

Indexed as

cardiac allograftHuman umbilical cord mesenchymal stromal cellsratvascular endothelia

Identifiers

PMID25126177
PMCPMC4132141

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.