ArticleAnalytical methods : advancing methods and applications2014
Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis.
Article in Analytical methods : advancing methods and applications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
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- Analytical Techniques for the Characterization and Quantification of Monoclonal Antibodies.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Protocol for high-throughput cloning, expression, purification, and evaluation of bispecific antibodies.STAR protocols · 2022Article
- Susceptibility of Beavers to Chronic Wasting Disease.Biology · 2022Article
- The Intriguing Landscape of Single-Cell Protein Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Capillary Electrophoresis-Mass Spectrometry (CE-MS) by Sheath-Flow Nanospray Interface and Its Use in Biopharmaceutical Applications.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Comparability of Biologics: Global Principles, Evidentiary Consistency and Unrealized Reliance.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021Article
- Antibody Conjugate Assembly on Ultrasound-Confined Microcarrier Particles.ACS biomaterials science & engineering · 2020Article
- Microfluidics as a Novel Tool for Biological and Toxicological Assays in Drug Discovery Processes: Focus on Microchip Electrophoresis.Micromachines · 2020Review
- Monitorization of α1-Acid Glycoprotein Deglycosylation Using SU-8 Microchips Electrophoresis with LIF Detection.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Review
- Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary.Journal of visualized experiments : JoVE · 2017Article
- Early Evidence of Low Bone Density and Decreased Serotonergic Synthesis in the Dorsal Raphe of a Tauopathy Model of Alzheimer's Disease.Journal of Alzheimer's disease : JAD · 2017Article
- Acupuncture Injection Combined with Electrokinetic Injection for Polydimethylsiloxane Microfluidic Devices.Journal of analytical methods in chemistry · 2017Article
- Recent Advances in Microscale Western Blotting.Analytical methods : advancing methods and applications · 2016Article
- Host Protein Biomarkers Identify Active Tuberculosis in HIV Uninfected and Co-infected Individuals.EBioMedicine · 2015Article
- Sustained high levels of neuroprotective, high molecular weight, phosphorylated tau in the longest-lived rodent.Neurobiology of aging · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The development of therapeutic proteins and peptides is an expensive and time-intensive process. Biologics, which have become a multi-billion dollar industry, are chemically complex products that require constant observation during each stage of development and production. Post-translational modifications along with chemical and physical degradation from oxidation, deamidation, and aggregation, lead to high levels of heterogeneity that affect drug quality and efficacy. The various separation modes of capillary electrophoresis (CE) are commonly utilized to perform quality control and assess protein heterogeneity. This review attempts to highlight the most recent developments and applications of CE separation techniques for the characterization of protein and peptide therapeutics by focusing on papers accepted for publication in the in the two-year period between January 2012 and December 2013. The separation principles and technological advances of CE, capillary gel electrophoresis, capillary isoelectric focusing, capillary electrochromatography and CE-mass spectrometry are discussed, along with exciting new applications of these techniques to relevant pharmaceutical issues. Also included is a small selection of papers on microchip electrophoresis to show the direction this field is moving with regards to the development of inexpensive and portable analysis systems for on-site, high-throughput analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.