ArticleInsect molecular biology2014
Transcription activator-like effector nuclease (TALEN)-mediated female-specific sterility in the silkworm, Bombyx mori.
Article in Insect molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 54 citations in OpenAlex.
- Identification of a high-activity and ubiquitous promoter from lepidoptera insect for transgenic applications.Journal of insect science (Online) · 2026Article
- Doublesex and GATAβ4 synergistically regulate the sex-dimorphic expression of storage protein 1 in Bombyx mori.PLoS genetics · 2025Article
- Transcriptomic Evidence for Cell-Autonomous Sex Differentiation of the Gynandromorphic Fat Body in the Silkworm,Journal of developmental biology · 2024Article
- CRISPR/Cas9-Mediated Mutagenesis of Sex-SpecificCells · 2022Article
- Identification and Expression Analysis ofInternational journal of molecular sciences · 2022Article
- Article
- Effects of Functional Depletion ofInsects · 2021Article
- The Sex Determination Cascade in the Silkworm.Genes · 2021Review
- Mini-Review Regarding the Applicability of Genome Editing Techniques Developed for Studying Infertility.Diagnostics (Basel, Switzerland) · 2021Review
- Transgenic and knockout analyses of Masculinizer and doublesex illuminated the unique functions of doublesex in germ cell sexual development of the silkworm, Bombyx mori.BMC developmental biology · 2020Article
- Regulation of olfactory-based sex behaviors in the silkworm by genes in the sex-determination cascade.PLoS genetics · 2020Article
- CRISPR Disruption ofInsects · 2019Article
- The C-terminus of DSX(F5) protein acts as a novel regulatory domain in Bombyx mori.Transgenic research · 2016Article
- Transgenic Expression of the piRNA-Resistant Masculinizer Gene Induces Female-Specific Lethality and Partial Female-to-Male Sex Reversal in the Silkworm, Bombyx mori.PLoS genetics · 2016Article
- Pheromone-Based Pest Management in China: Past, Present, and Future Prospects.Journal of chemical ecology · 2016Review
- Genome Editing ofFrontiers in physiology · 2016Article
- siRNA-Mediated Silencing of doublesex during Female Development of the Dengue Vector Mosquito Aedes aegypti.PLoS neglected tropical diseases · 2015Article
- Advanced technologies for genetically manipulating the silkworm Bombyx mori, a model Lepidopteran insect.Proceedings. Biological sciences · 2015Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Engineering sex-specific sterility is critical for developing transgene-based sterile insect technology. Targeted genome engineering achieved by customized zinc-finger nuclease, transcription activator-like effector nuclease (TALEN) or clustered, regularly interspaced, short palindromic repeats/Cas9 systems has been exploited extensively in a variety of model organisms; however, screening mutated individuals without a detectable phenotype is still challenging. In addition, genetically recessive mutations only detectable in homozygotes make the experiments time-consuming. In the present study, we model a novel genetic system in the silkworm, Bombyx mori, that results in female-specific sterility by combining transgenesis with TALEN technologies. This system induces sex-specific sterility at a high efficiency by targeting the female-specific exon of the B. mori doublesex (Bmdsx) gene, which has sex-specific splicing isoforms regulating somatic sexual development. Transgenic animals co-expressing TALEN left and right arms targeting the female-specific Bmdsx exon resulted in somatic mutations and female mutants lost fecundity because of lack of egg storage and abnormal external genitalia. The wild-type sexual dimorphism of abdominal segment was not evident in mutant females. In contrast, there were no deleterious effects in mutant male moths. The current somatic TALEN technologies provide a promising approach for future insect functional genetics, thus providing the basis for the development of attractive genetic alternatives for insect population management.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.