Evidence map›Paper›PMID 25124286›Full record

ArticleComputerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society2014

An image processing approach to analyze morphological features of microscopic images of muscle fibers.

Cesar Henrique Comin, Xiaoyin Xu, Yaming Wang, Luciano da Fontoura Costa, Zhong Yang

Open access · greenAbstract read
In one paragraph

Article in Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 3 countries.

Cesar Henrique CominUniversity of Sao Paulo, Institute of Physics at Sao Carlos, Sao Paulo, Brazil.
Xiaoyin XuDepartment of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Yaming WangDepartment of Anesthesia, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Luciano da Fontoura CostaUniversity of Sao Paulo, Institute of Physics at Sao Carlos, Sao Paulo, Brazil.
Zhong YangDepartment of Clinical Hematology, The Third Military Medical University, Chongqing, China. Electronic address: zyang@tmmu.edu.cn.
Universidade de São Paulo · BRArmy Medical University · CNBrigham and Women's Hospital · USHarvard University · US

Funding

A novel informatics approach to understanding complex muscle fiber phenotypesR01LM011415 · NLM · BRIGHAM AND WOMEN'S HOSPITAL · PI XU, XIAOYIN · 2014 to 2017
$1.5M
Strategies to improve stem cell engraftment into muscleK02AR051181 · NIAMS · BRIGHAM AND WOMEN'S HOSPITAL · PI WANG, YAMING · 2004 to 2008
$535k
NIAMS NIH HHS K02 AR051181NLM NIH HHS R01 LM011415
6 · The paper itself

Abstract

We present an image processing approach to automatically analyze duo-channel microscopic images of muscular fiber nuclei and cytoplasm. Nuclei and cytoplasm play a critical role in determining the health and functioning of muscular fibers as changes of nuclei and cytoplasm manifest in many diseases such as muscular dystrophy and hypertrophy. Quantitative evaluation of muscle fiber nuclei and cytoplasm thus is of great importance to researchers in musculoskeletal studies. The proposed computational approach consists of steps of image processing to segment and delineate cytoplasm and identify nuclei in two-channel images. Morphological operations like skeletonization is applied to extract the length of cytoplasm for quantification. We tested the approach on real images and found that it can achieve high accuracy, objectivity, and robustness.

Indexed as

AlgorithmsAnimalsCells, CulturedImage EnhancementImage Interpretation, Computer-AssistedMaleMiceMice, Inbred C57BLMicroscopyMuscle Fibers, SkeletalPattern Recognition, AutomatedReproducibility of ResultsSensitivity and SpecificityCytoplasmMuscle fiberNucleiQuantificationSegmentationSkeletonization

Identifiers

PMID25124286
PMCPMC4955614
OpenAlexW2029331714

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.