ArticleMethods (San Diego, Calif.)2014
A quantitative multiplexed mass spectrometry assay for studying the kinetic of residue-specific histone acetylation.
Article in Methods (San Diego, Calif.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Acetylcarnitine shuttling links mitochondrial metabolism to histone acetylation and lipogenesis.Science advances · 2023Article
- Surprising phenotypic diversity of cancer-associated mutations of Gly 34 in the histone H3 tail.eLife · 2021Article
- Histone acylation marks respond to metabolic perturbations and enable cellular adaptation.Experimental & molecular medicine · 2020Article
- Regulation of plant architecture by a new histone acetyltransferase targeting gene bodies.Nature plants · 2020Article
- Discordant Effects of Putative Lysine Acetyltransferase Inhibitors in Biochemical and Living Systems.Cells · 2019Article
- Two factor authentication: Asf1 mediates crosstalk between H3 K14 and K56 acetylation.Nucleic acids research · 2019Article
- IL-35 (Interleukin-35) Suppresses Endothelial Cell Activation by Inhibiting Mitochondrial Reactive Oxygen Species-Mediated Site-Specific Acetylation of H3K14 (Histone 3 Lysine 14).Arteriosclerosis, thrombosis, and vascular biology · 2018Article
- LC-MS/MS-based quantitative study of the acyl group- and site-selectivity of human sirtuins to acylated nucleosomes.Scientific reports · 2018Article
- Article
- Overlapping and Divergent Actions of Structurally Distinct Histone Deacetylase Inhibitors in Cardiac Fibroblasts.The Journal of pharmacology and experimental therapeutics · 2017Article
- Impact of a High-fat Diet on Tissue Acyl-CoA and Histone Acetylation Levels.The Journal of biological chemistry · 2017Article
- Interaction with the DNA Repair Protein Thymine DNA Glycosylase Regulates Histone Acetylation by p300.Biochemistry · 2016Article
- Measuring specificity in multi-substrate/product systems as a tool to investigate selectivity in vivo.Biochimica et biophysica acta · 2016Review
- Site specificity analysis of Piccolo NuA4-mediated acetylation for different histone complexes.The Biochemical journal · 2015Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
Histone acetylation is involved in gene regulation and, most importantly, aberrant regulation of histone acetylation is correlated with major human diseases. Although many lysine acetyltransferases (KATs) have been characterized as being capable of acetylating multiple lysine residues on histones, how different factors such as enzyme complexes or external stimuli (e.g. KAT activators or inhibitors) alter KAT specificity remains elusive. In order to comprehensively understand how the homeostasis of histone acetylation is maintained, a method that can quantitate acetylation levels of individual lysines on histones is needed. Here we demonstrate that our mass spectrometry (MS)-based method accomplishes this goal. In addition, the high throughput, high sensitivity, and high dynamic range of this method allows for effectively and accurately studying steady-state kinetics. Based on the kinetic parameters from in vitro enzymatic assays, we can determine the specificity and selectivity of a KAT and use this information to understand what factors influence histone acetylation. These approaches can be used to study the enzymatic mechanisms of histone acetylation as well as be adapted to other histone modifications. Understanding the post-translational modification of individual residues within the histones will provide a better picture of chromatin regulation in the cell.
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