ReviewMicrobial biotechnology2015
Synthetic biology approaches to improve biocatalyst identification in metagenomic library screening.
Review in Microbial biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 73 citations in OpenAlex.
- Strategies, Achievements, and Potential Challenges of Plant and Microbial Chassis in the Biosynthesis of Plant Secondary Metabolites.Molecules (Basel, Switzerland) · 2024Review
- Functional Metagenomics as a Tool to Tap into Natural Diversity of Valuable Biotechnological Compounds.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Identification and functional analysis of novel protein-encoding sequences related to stress-resistance.Frontiers in microbiology · 2023Article
- Article
- Stabilization of Enzymes by Using Thermophiles.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Tuning Immobilized Enzyme Features by Combining Solid-Phase Physicochemical Modification and Mineralization.International journal of molecular sciences · 2022Article
- Marine Bioprospecting, Biocatalysis and Process Development.Microorganisms · 2022Review
- Bioprospecting for Thermozymes and Characterization of a Novel Lipolytic Thermozyme Belonging to the SGNH/GDSL Family of Hydrolases.International journal of molecular sciences · 2022Article
- Boundaries in metagenomic screenings using lacZα-based vectors.Genetics and molecular biology · 2020Article
- Emerging strategies for the development of food industries.Bioengineered · 2019Review
- Genetically Engineered Proteins to Improve Biomass Conversion: New Advances and Challenges for Tailoring Biocatalysts.Molecules (Basel, Switzerland) · 2019Review
- Recovery and functional validation of hidden soil enzymes in metagenomic libraries.MicrobiologyOpen · 2019Article
- The art of vector engineering: towards the construction of next-generation genetic tools.Microbial biotechnology · 2019Review
- Boosting Secondary Metabolite Production and Discovery through the Engineering of Novel Microbial Biosensors.BioMed research international · 2018Review
- Novel Ethanol- and 5-Hydroxymethyl Furfural-Stimulated β-Glucosidase Retrieved From a Brazilian Secondary Atlantic Forest Soil Metagenome.Frontiers in microbiology · 2018Article
- Metagenomic Approaches for Understanding New Concepts in Microbial Science.International journal of genomics · 2018Review
- Mining Novel Constitutive Promoter Elements in Soil Metagenomic Libraries inFrontiers in microbiology · 2018Article
- A metagenome-derived thermostable β-glucanase with an unusual module architecture which defines the new glycoside hydrolase family GH148.Scientific reports · 2017Article
- Synthetic extreme environments: overlooked sources of potential biotechnologically relevant microorganisms.Microbial biotechnology · 2017Article
- Whole cell biocatalysts: essential workers from Nature to the industry.Microbial biotechnology · 2017Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is a growing demand for enzymes with improved catalytic performance or tolerance to process-specific parameters, and biotechnology plays a crucial role in the development of biocatalysts for use in industry, agriculture, medicine and energy generation. Metagenomics takes advantage of the wealth of genetic and biochemical diversity present in the genomes of microorganisms found in environmental samples, and provides a set of new technologies directed towards screening for new catalytic activities from environmental samples with potential biotechnology applications. However, biased and low level of expression of heterologous proteins in Escherichia coli together with the use of non-optimal cloning vectors for the construction of metagenomic libraries generally results in an extremely low success rate for enzyme identification. The bottleneck arising from inefficient screening of enzymatic activities has been addressed from several perspectives; however, the limitations related to biased expression in heterologous hosts cannot be overcome by using a single approach, but rather requires the synergetic implementation of multiple methodologies. Here, we review some of the principal constraints regarding the discovery of new enzymes in metagenomic libraries and discuss how these might be resolved by using synthetic biology methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.