ReviewMolecules (Basel, Switzerland)2014
Imaging live cells at the nanometer-scale with single-molecule microscopy: obstacles and achievements in experiment optimization for microbiology.
Review in Molecules (Basel, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Effect of shear rate on earlyBiofilm · 2024Article
- Independent Promoter Recognition by TcpP Precedes Cooperative Promoter Activation by TcpP and ToxR.mBio · 2021Article
- Computerized fluorescence microscopy of microbial cells.World journal of microbiology & biotechnology · 2021Review
- Cellular dynamics of the SecA ATPase at the single molecule level.Scientific reports · 2021Article
- Rhenium (I) Complexes as Probes for Prokaryotic and Fungal Cells by Fluorescence Microscopy: Do Ligands Matter?Frontiers in chemistry · 2019Review
- EzColocalization: An ImageJ plugin for visualizing and measuring colocalization in cells and organisms.Scientific reports · 2018Article
- Bacterial cell wall nanoimaging by autoblinking microscopy.Scientific reports · 2018Article
- Microdomain formation is a general property of bacterial membrane proteins and induces heterogeneity of diffusion patterns.BMC biology · 2018Article
- Intracellular localization of nanoparticle dimers by chirality reversal.Nature communications · 2017Article
- Resolving Fast, Confined Diffusion in Bacteria with Image Correlation Spectroscopy.Biophysical journal · 2016Article
- Addressing the Requirements of High-Sensitivity Single-Molecule Imaging of Low-Copy-Number Proteins in Bacteria.Chemphyschem : a European journal of chemical physics and physical chemistry · 2016Article
- Special issue: single molecule techniques.Molecules (Basel, Switzerland) · 2015Article
- Single-molecule tracking in live Vibrio cholerae reveals that ToxR recruits the membrane-bound virulence regulator TcpP to the toxT promoter.Molecular microbiology · 2015Article
- Molecular approaches to chromatography using single molecule spectroscopy.Analytical chemistry · 2015Review
- Super-Resolution Scanning Laser Microscopy Based on Virtually Structured Detection.Critical reviews in biomedical engineering · 2015Review
- Enhanced fluorescence emitted by microdroplets containing organic dye emulsions.Biomicrofluidics · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Single-molecule fluorescence microscopy enables biological investigations inside living cells to achieve millisecond- and nanometer-scale resolution. Although single-molecule-based methods are becoming increasingly accessible to non-experts, optimizing new single-molecule experiments can be challenging, in particular when super-resolution imaging and tracking are applied to live cells. In this review, we summarize common obstacles to live-cell single-molecule microscopy and describe the methods we have developed and applied to overcome these challenges in live bacteria. We examine the choice of fluorophore and labeling scheme, approaches to achieving single-molecule levels of fluorescence, considerations for maintaining cell viability, and strategies for detecting single-molecule signals in the presence of noise and sample drift. We also discuss methods for analyzing single-molecule trajectories and the challenges presented by the finite size of a bacterial cell and the curvature of the bacterial membrane.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.