ArticleThe Journal of cell biology1989
Association of ligand-receptor complexes with actin filaments in human neutrophils: a possible regulatory role for a G-protein.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 70 citations in OpenAlex.
- Developmental expression of the alpha-skeletal actin gene.BMC evolutionary biology · 2008Article
- Two pathways through Cdc42 couple the N-formyl receptor to actin nucleation in permeabilized human neutrophils.The Journal of cell biology · 2000Article
- Role of gelsolin in actin depolymerization of adherent human neutrophils.Molecular biology of the cell · 1997Article
- Activation of phospholipase D is an early event in integrin-mediated signalling leading to phagocytosis in human neutrophils.Inflammation · 1996Article
- Leukotriene D4-induced mobilization of intracellular Ca2+ in epithelial cells is critically dependent on activation of the small GTP-binding protein Rho.The Biochemical journal · 1996Article
- Chemoattractant-induced NADPH oxidase activity in human monocytes is terminated without any association of receptor-ligand complex to cytoskeleton.Inflammation · 1995Article
- Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes.Molecular biology of the cell · 1995Article
- Sequences responsible for intracellular localization of beta-actin messenger RNA also affect cell phenotype.The Journal of cell biology · 1994Article
- Glycoprotein IIb-IIIa and the translocation of Rap2B to the platelet cytoskeleton.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Beta 2 integrin engagement triggers actin polymerization and phosphatidylinositol trisphosphate formation in non-adherent human neutrophils.The Journal of cell biology · 1993Article
- Stimulus-induced dissociation of alpha subunits of heterotrimeric GTP-binding proteins from the cytoskeleton of human neutrophils.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Article
- N-formyl peptide receptors in human neutrophils display distinct membrane distribution and lateral mobility when labeled with agonist and antagonist.The Journal of cell biology · 1993Article
- A GTPase controls cell-substrate adhesion in Xenopus XTC fibroblasts.The Journal of cell biology · 1992Article
- The cytoskeleton and its importance as a mediator of inflammation.Annals of the rheumatic diseases · 1992Review
- Tumor cell adhesion to the extracellular matrix and signal transduction mechanisms implicated in tumor cell motility, invasion and metastasis.Cancer metastasis reviews · 1992Review
- Stimulus-response uncoupling in the neutrophil. Adenosine A2-receptor occupancy inhibits the sustained, but not the early, events of stimulus transduction in human neutrophils by a mechanism independent of actin-filament formation.The Biochemical journal · 1992Article
- Article
- Down-regulation of receptor antigen in leukotriene B4-induced chemotactic deactivation of human polymorphonuclear leucocytes.Immunology · 1991Article
- Octyl glucoside extracts GTP-binding regulatory proteins from rat brain "synaptoneurosomes" as large, polydisperse structures devoid of beta gamma complexes and sensitive to disaggregation by guanine nucleotides.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most ligand-receptor interactions result in an immediate generation of various second messengers and a subsequent association of the ligand-receptor complex to the cytoskeleton. Depending on the receptor involved, this linkage to the cytoskeleton has been suggested to play a role in the termination of second messenger generation and/or the endocytic process whereby the ligand-receptor complex is internalized. We have studied how the binding of chemotactic peptide-receptor complexes to the cytoskeleton of human neutrophils is accomplished. As much as 76% of the tritiated formylmethionyl-leucyl-phenylalanine (fMet-Leu-[3H]Phe) specifically bound to intact cells, obtained by a 30-s stimulation with 20 nM fMet-Leu-[3H]Phe, still remained after Triton X-100 extraction. Preincubating intact cells with dihydrocytochalasin B (dhCB) or washing the cytoskeletal preparation with a high concentration of potassium, reduced the binding of ligand-receptor complexes to the cytoskeleton by 46% or more. Inhibition of fMet-Leu-Phe-induced generation of second messengers by ADP-ribosylating the alpha-subunit of the receptor-coupled G-protein with pertussis toxin, did not reduce the binding of ligand-receptor complexes to the cytoskeleton. However, using guanosine-5'-O-(2-thiodiphosphate) (GDP beta S) to prevent the dissociation of the fMet-Leu-Phe-associated G-protein within electrically permeabilized cells, led to a pronounced reduction (62%) of the binding between ligand-receptor complexes and the cytoskeleton. In summary, in human neutrophils the rapid association between chemotactic peptide-receptor complexes and the cytoskeleton is dependent on filamentous actin. This association is most likely regulated by the activation and dissociation of the fMet-Leu-Phe-associated G-protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.