ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2014
Exocytotic machineries of vestibular type I and cochlear ribbon synapses display similar intrinsic otoferlin-dependent Ca2+ sensitivity but a different coupling to Ca2+ channels.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 61 citations in OpenAlex.
- Transient capacitance changes recorded from vestibular type I hair cells are produced by GThe Journal of physiology · 2025Article
- Auditory Hair Cells and Spiral Ganglion Neurons Regenerate Synapses with Refined Release Properties In Vitro.bioRxiv : the preprint server for biology · 2023Article
- mTORC2 regulates auditory hair cell structure and function.iScience · 2023Article
- Otoferlin as a multirole CaFrontiers in cellular neuroscience · 2023Review
- Age-dependent structural reorganization of utricular ribbon synapses.Frontiers in cell and developmental biology · 2023Article
- The SNARE protein SNAP-25 is required for normal exocytosis at auditory hair cell ribbon synapses.iScience · 2022Article
- Loss of Pex1 in Inner Ear Hair Cells Contributes to Cochlear Synaptopathy and Hearing Loss.Cells · 2022Article
- Signal transmission in mature mammalian vestibular hair cells.Frontiers in cellular neuroscience · 2022Article
- A sensory cell diversifies its output by varying CaThe EMBO journal · 2021Article
- Current Response in CaFrontiers in neuroscience · 2021Article
- Synaptic Release Potentiation at Aging Auditory Ribbon Synapses.Frontiers in aging neuroscience · 2021Article
- Activation of GABAJournal of neurophysiology · 2020Article
- Exocytosis in mouse vestibular Type II hair cells shows a high-order CaPhysiological reports · 2020Article
- KNeuroscience · 2020Article
- Protection of Cochlear Ribbon Synapses and Prevention of Hidden Hearing Loss.Neural plasticity · 2020Review
- Uncoordinated maturation of developing and regenerating postnatal mammalian vestibular hair cells.PLoS biology · 2019Article
- Phase Locking of Auditory-Nerve Fibers Reveals Stereotyped Distortions and an Exponential Transfer Function with a Level-Dependent Slope.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Viral Transfer of Mini-Otoferlins Partially Restores the Fast Component of Exocytosis and Uncovers Ultrafast Endocytosis in Auditory Hair Cells of Otoferlin Knock-Out Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Clustered CaCell reports · 2018Article
- Vesicle sub-pool organization at inner hair cell ribbon synapses.EMBO reports · 2018Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hair cell ribbon synapses of the mammalian auditory and vestibular systems differ greatly in their anatomical organization and firing properties. Notably, vestibular Type I hair cells (VHC-I) are surrounded by a single calyx-type afferent terminal that receives input from several ribbons, whereas cochlear inner hair cells (IHCs) are contacted by several individual afferent boutons, each facing a single ribbon. The specificity of the presynaptic molecular mechanisms regulating transmitter release at these different sensory ribbon synapses is not well understood. Here, we found that exocytosis during voltage activation of Ca(2+) channels displayed higher Ca(2+) sensitivity, 10 mV more negative half-maximum activation, and a smaller dynamic range in VHC-I than in IHCs. VHC-I had a larger number of Ca(2+) channels per ribbon (158 vs 110 in IHCs), but their Ca(2+) current density was twofold smaller because of a smaller open probability and unitary conductance. Using confocal and stimulated emission depletion immunofluorescence microscopy, we showed that VHC-I had fewer synaptic ribbons (7 vs 17 in IHCs) to which Cav1.3 channels are more tightly organized than in IHCs. Gradual intracellular Ca(2+) uncaging experiments revealed that exocytosis had a similar intrinsic Ca(2+) sensitivity in both VHC-I and IHCs (KD of 3.3 ± 0.6 μM and 4.0 ± 0.7 μM, respectively). In otoferlin-deficient mice, exocytosis was largely reduced in VHC-I and IHCs. We conclude that VHC-I and IHCs use a similar micromolar-sensitive otoferlin Ca(2+) sensor and that their sensory encoding specificity is essentially determined by a different functional organization of Ca(2+) channels at their synaptic ribbons.
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