ArticleThe Journal of biological chemistry2014
A pituitary homeobox 2 (Pitx2):microRNA-200a-3p:β-catenin pathway converts mesenchymal cells to amelogenin-expressing dental epithelial cells.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Immunohistochemical Expression of CK13 and Molecular Analysis of KRT13 and APC in Odontogenic Ghost Cell Lesions, Adenoid Ameloblastoma, and Conventional Ameloblastoma.Head and neck pathology · 2026Article
- Activation of developmental transcription factors using RNA technology promotes heart repair.bioRxiv : the preprint server for biology · 2026Article
- miR-200a TargetsAnimals : an open access journal from MDPI · 2025Article
- Inhibition of craniosynostosis and premature suture fusion inScience advances · 2025Article
- Single-nuclei multiomics analysis identifies abnormal cardiomyocytes in a murine model of cardiac development.Nature communications · 2025Article
- RNA Technology to Regenerate and Repair Alveolar Bone Defects.Journal of dental research · 2024Article
- Exploring microRNAs in craniofacial regenerative medicine.Biochemical Society transactions · 2023Review
- Periosteal reaction as a crucial radiographic finding for desmoplastic fibroma of the jaw bone in children: A case report.Imaging science in dentistry · 2022Article
- The miR-200 family is required for ectodermal organ development through the regulation of the epithelial stem cell niche.Stem cells (Dayton, Ohio) · 2021Article
- PRRX1 deficiency induces mesenchymal-epithelial transition through PITX2/miR-200-dependent SLUG/CTNNB1 regulation in hepatocellular carcinoma.Cancer science · 2021Article
- Disease Modeling and Disease Gene Discovery in Cardiomyopathies: A Molecular Study of Induced Pluripotent Stem Cell Generated Cardiomyocytes.International journal of molecular sciences · 2021Article
- New insight into dental epithelial stem cells: Identification, regulation, and function in tooth homeostasis and repair.World journal of stem cells · 2020Review
- ATRA induces the differentiation of hepatic progenitor cells by upregulating microRNA-200a.In vitro cellular & developmental biology. Animal · 2019Article
- Primordial odontogenic tumor: a case report and literature review.Diagnostic pathology · 2019Review
- PITX2 Expression in Non-functional Pituitary Neuroendocrine Tumor with Cavernous Sinus Invasion.Endocrine pathology · 2019Article
- The TFAP2A-IRF6-GRHL3 genetic pathway is conserved in neurulation.Human molecular genetics · 2019Article
- WIF1 enhanced dentinogenic differentiation in stem cells from apical papilla.BMC oral health · 2019Article
- Mandible exosomal ssc-mir-133b regulates tooth development in miniature swine via endogenous apoptosis.Bone research · 2018Article
- Wnt and BMP Signaling Crosstalk in Regulating Dental Stem Cells: Implications in Dental Tissue Engineering.Genes & diseases · 2016Article
- MicroRNA 665 Regulates Dentinogenesis through MicroRNA-Mediated Silencing and Epigenetic Mechanisms.Molecular and cellular biology · 2015Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Pitx2, Wnt/β-catenin signaling, and microRNAs (miRs) play a critical role in the regulation of dental stem cells during embryonic development. In this report, we have identified a Pitx2:β-catenin regulatory pathway involved in epithelial cell differentiation and conversion of mesenchymal cells to amelogenin expressing epithelial cells via miR-200a. Pitx2 and β-catenin are expressed in the labial incisor cervical loop or epithelial stem cell niche, with decreased expression in the differentiating ameloblast cells of the mouse lower incisor. Bioinformatics analyses reveal that miR-200a-3p expression is activated in the pre-ameloblast cells to enhance epithelial cell differentiation. We demonstrate that Pitx2 activates miR-200a-3p expression and miR-200a-3p reciprocally represses Pitx2 and β-catenin expression. Pitx2 and β-catenin interact to synergistically activate gene expression during odontogenesis and miR-200a-3p attenuates their expression and directs differentiation. To understand how this mechanism controls cell differentiation and cell fate, oral epithelial and odontoblast mesenchymal cells were reprogrammed by a two-step induction method using Pitx2 and miR-200a-3p. Conversion to amelogenin expressing dental epithelial cells involved an up-regulation of the stem cell marker Sox2 and proliferation genes and decreased expression of mesenchymal markers. E-cadherin expression was increased as well as ameloblast specific factors. The combination of Pitx2, a regulator of dental stem cells and miR-200a converts mesenchymal cells to a fully differentiated dental epithelial cell type. This pathway and reprogramming can be used to reprogram mesenchymal or oral epithelial cells to dental epithelial (ameloblast) cells, which can be used in tissue repair and regeneration studies.
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