ArticleNucleic acids research2014
Systematic exploration of a class of hydrophobic unnatural base pairs yields multiple new candidates for the expansion of the genetic alphabet.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Article
- Reverse transcription as key step in RNARSC chemical biology · 2024Article
- Engineering tRNAs for the Ribosomal Translation of Non-proteinogenic Monomers.Chemical reviews · 2024Review
- Detecting Hachimoji DNA: An Eight-Building-Block Genetic System with MoSACS applied materials & interfaces · 2024Article
- Stability of nucleic acid bases in concentrated sulfuric acid: Implications for the habitability of Venus' clouds.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Complementary base pair interactions between different rare tautomers of the second-generation artificial genetic alphabets.Journal of molecular modeling · 2023Article
- In vitro selection of aptamers and their applications.Nature reviews. Methods primers · 2023Article
- From polymerase engineering to semi-synthetic life: artificial expansion of the central dogma.RSC chemical biology · 2022Review
- Replacing thymine with a strongly pairing fifth Base: A combined quantum mechanics and molecular dynamics study.Computational and structural biotechnology journal · 2021Article
- Synthetic Biological Circuits within an Orthogonal Central Dogma.Trends in biotechnology · 2021Review
- Posttranscriptional site-directed spin labeling of large RNAs with an unnatural base pair system under non-denaturing conditions.Chemical science · 2020Article
- Strategic labelling approaches for RNA single-molecule spectroscopy.RNA biology · 2019Review
- Optimization of Replication, Transcription, and Translation in a Semi-Synthetic Organism.Journal of the American Chemical Society · 2019Article
- Next-generation genetic code expansion.Current opinion in chemical biology · 2018Review
- In Vivo Structure-Activity Relationships and Optimization of an Unnatural Base Pair for Replication in a Semi-Synthetic Organism.Journal of the American Chemical Society · 2017Article
- Helix instability and self-pairing prevent unnatural base pairs from expanding the genetic alphabet.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Chemical Stabilization of Unnatural Nucleotide Triphosphates for the in Vivo Expansion of the Genetic Alphabet.Journal of the American Chemical Society · 2017Article
- A semisynthetic organism engineered for the stable expansion of the genetic alphabet.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Evolvability Is an Evolved Ability: The Coding Concept as the Arch-Unit of Natural Selection.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2016Article
- Overcoming Challenges in Engineering the Genetic Code.Journal of molecular biology · 2016Review
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Authors and funding
6 authors.
Funding
Abstract
We have developed a family of unnatural base pairs (UBPs), which rely on hydrophobic and packing interactions for pairing and which are well replicated and transcribed. While the pair formed between d5SICS and dNaM (d5SICS-dNaM) has received the most attention, and has been used to expand the genetic alphabet of a living organism, recent efforts have identified dTPT3-dNaM, which is replicated with even higher fidelity. These efforts also resulted in more UBPs than could be independently analyzed, and thus we now report a PCR-based screen to identify the most promising. While we found that dTPT3-dNaM is generally the most promising UBP, we identified several others that are replicated nearly as well and significantly better than d5SICS-dNaM, and are thus viable candidates for the expansion of the genetic alphabet of a living organism. Moreover, the results suggest that continued optimization should be possible, and that the putatively essential hydrogen-bond acceptor at the position ortho to the glycosidic linkage may not be required. These results clearly demonstrate the generality of hydrophobic forces for the control of base pairing within DNA, provide a wealth of new structure-activity relationship data and importantly identify multiple new candidates for in vivo evaluation and further optimization.
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