ArticleNature protocols2014
Preparation of microcrystals in lipidic cubic phase for serial femtosecond crystallography.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed.
- Advancing macromolecular structure determination with microsecond X-ray pulses at a 4th generation synchrotron.Communications chemistry · 2025Article
- Review of serial femtosecond crystallography including the COVID-19 pandemic impact and future outlook.Structure (London, England : 1993) · 2023Review
- A versatile approach to high-density microcrystals in lipidic cubic phase for room-temperature serial crystallography.Journal of applied crystallography · 2023Article
- Structure-based drug discovery of a corticotropin-releasing hormone receptor 1 antagonist using an X-ray free-electron laser.Experimental & molecular medicine · 2023Article
- Dynamic catcher for stabilization of high-viscosity extrusion jets.Journal of applied crystallography · 2023Article
- Growing and making nano- and microcrystals.Nature protocols · 2023Review
- Serial femtosecond crystallography.Nature reviews. Methods primers · 2022Article
- Beef tallow injection matrix for serial crystallography.Scientific reports · 2022Article
- Crystallization of Microbial Rhodopsins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography.Lab on a chip · 2021Article
- MicroED structure of the human adenosine receptor determined from a single nanocrystal in LCP.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Molecular basis for lipid recognition by the prostaglandin DProceedings of the National Academy of Sciences of the United States of America · 2021Article
- MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography.Nature communications · 2021Article
- Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures.Journal of applied crystallography · 2020Article
- Small-wedge synchrotron and serial XFEL datasets for Cysteinyl leukotriene GPCRs.Scientific data · 2020Article
- Isoprenoid-chained lipid EROCOCScientific reports · 2020Article
- Article
- Structure Determination from Lipidic Cubic Phase Embedded Microcrystals by MicroED.Structure (London, England : 1993) · 2020Article
- Lard Injection Matrix for Serial Crystallography.International journal of molecular sciences · 2020Article
- Article
16 more citing papers are in PubMed but not listed here.
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3 authors.
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Abstract
We have recently established a procedure for serial femtosecond crystallography (SFX) in lipidic cubic phase (LCP) for protein structure determination at X-ray free-electron lasers (XFELs). LCP-SFX uses the gel-like LCP as a matrix for growth and delivery of membrane protein microcrystals for crystallographic data collection. LCP is a liquid-crystalline mesophase composed of lipids and water. It provides a membrane-mimicking environment that stabilizes membrane proteins and supports their crystallization. Here we describe detailed procedures for the preparation and characterization of microcrystals for LCP-SFX applications. The advantages of LCP-SFX over traditional crystallographic methods include the capability of collecting room-temperature high-resolution data with minimal effects of radiation damage from sub-10-μm crystals of membrane and soluble proteins that are difficult to crystallize, while eliminating the need for crystal harvesting and cryo-cooling. Compared with SFX methods for microcrystals in solution using liquid injectors, LCP-SFX reduces protein consumption by 2-3 orders of magnitude for data collection at currently available XFELs. The whole procedure typically takes 3-5 d, including the time required for the crystals to grow.
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