Evidence map›Paper›PMID 25121761›Full record

ArticlePloS one2014

Isolation of cancer stem cells from three human glioblastoma cell lines: characterization of two selected clones.

Fortunata Iacopino, Cristiana Angelucci, Roberto Piacentini, Filippo Biamonte, Annunziato Mangiola, Giulio Maira, Claudio Grassi, Gigliola Sica

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
3.4field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 63 citations in OpenAlex.

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  15. circPTN sponges miR-145-5p/miR-330-5p to promote proliferation and stemness in glioma.Journal of experimental & clinical cancer research : CR · 2019
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4 · The record

Corrections and comments

  • Erratum issued
5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Fortunata IacopinoInstitute of Histology and Embryology, Medical School, Catholic University of the Sacred Heart, Rome, Italy.
Cristiana AngelucciInstitute of Histology and Embryology, Medical School, Catholic University of the Sacred Heart, Rome, Italy.
Roberto PiacentiniInstitute of Human Physiology, Medical School, Catholic University of the Sacred Heart, Rome, Italy.
Filippo BiamonteInstitute of Histology and Embryology, Medical School, Catholic University of the Sacred Heart, Rome, Italy.
Annunziato MangiolaInstitute of Neurosurgery, Medical School, Catholic University of the Sacred Heart, Rome, Italy.
Giulio MairaInstitute of Neurosurgery, Medical School, Catholic University of the Sacred Heart, Rome, Italy.
Claudio GrassiInstitute of Human Physiology, Medical School, Catholic University of the Sacred Heart, Rome, Italy.
Gigliola SicaInstitute of Histology and Embryology, Medical School, Catholic University of the Sacred Heart, Rome, Italy.
Università Cattolica del Sacro Cuore · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cancer stem cells (CSC) were isolated via a non-adherent neurosphere assay from three glioma cell lines: LI, U87, and U373. Using a clonal assay, two clones (D2 and F11) were selected from spheres derived from LI cells and were characterized for the: expression of stem cell markers (CD133, Nestin, Musashi-1 and Sox2); proliferation; differentiation capability (determined by the expression of GalC, βIII-Tubulin and GFAP); Ca(2+) signaling and tumorigenicity in nude mice. Both D2 and F11 clones expressed higher levels of all stem cell markers with respect to the parental cell line. Clones grew more slowly than LI cells with a two-fold increase in duplication time. Markers of differentiation (βIII-Tubulin and GFAP) were expressed at high levels in both LI cells and in neurospheres. The expression of Nestin, Sox2, and βIII-Tubulin was down-regulated in D2 and F11 when cultured in serum-containing medium, whereas Musashi-1 was increased. In this condition, duplication time of D2 and F11 increased without reaching that of LI cells. D2, F11 and parental cells did not express voltage-dependent Ca(2+)-channels but they exhibited increased intracellular Ca(2+) levels in response to ATP. These Ca(2+) signals were larger in LI cells and in spheres cultured in serum-containing medium, while they were smaller in serum-free medium. The ATP treatment did not affect cell proliferation. Both D2 and F11 induced the appearance of tumors when ortotopically injected in athymic nude mice at a density 50-fold lower than that of LI cells. All these data indicate that both clones have characteristics of CSC and share the same stemness properties. The findings regarding the expression of differentiation markers and Ca(2+)-channels show that both clones are unable to reach the terminal differentiation. Both D2 and F11 might represent a good model to improve the knowledge on CSC in glioblastoma and to identify new therapeutic approaches.

Indexed as

Clone CellsAnimalsBiomarkers, TumorBlotting, WesternBrain NeoplasmsCell DifferentiationCell Line, TumorCulture MediaFlow CytometryGlioblastomaHumansMiceMice, NudeNeoplastic Stem CellsBiomarkers, TumorCulture Media

Identifiers

PMID25121761
PMCPMC4133365
OpenAlexW2014795303

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.