ArticlePLoS genetics2014
Phosphorylation of a central clock transcription factor is required for thermal but not photic entrainment.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Post-Translational Modifications in Animal Circadian Clocks.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Disordered but rhythmic-the role of intrinsic protein disorder in eukaryotic circadian timing.FEBS letters · 2026Review
- Alternative splicing ofProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Alternative splicing ofbioRxiv : the preprint server for biology · 2024Article
- CRUMB: a shiny-based app to analyze rhythmic feeding inF1000Research · 2023Article
- Article
- Roles of peripheral clocks: lessons from the fly.FEBS letters · 2022Review
- Peripheral Sensory Organs Contribute to Temperature Synchronization of the Circadian Clock inFrontiers in physiology · 2021Review
- The Phospho-Code Determining Circadian Feedback Loop Closure and Output in Neurospora.Molecular cell · 2019Article
- AMP-Activated Protein Kinase Regulates Circadian Rhythm by Affecting CLOCK inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- A simple method to measure CLOCK-BMAL1 DNA binding activity in tissue and cell extracts.F1000Research · 2017Article
- Cycles of circadian illuminance are sufficient to entrain and maintain circadian locomotor rhythms in Drosophila.Scientific reports · 2016Article
- Pacemaker-neuron-dependent disturbance of the molecular clockwork by a Drosophila CLOCK mutant homologous to the mouse Clock mutation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Protein sequestration versus Hill-type repression in circadian clock models.IET systems biology · 2016Review
- Identification of Light-Sensitive Phosphorylation Sites on PERIOD That Regulate the Pace of Circadian Rhythms in Drosophila.Molecular and cellular biology · 2015Article
- Article
- The molecular ticks of the Drosophila circadian clock.Current opinion in insect science · 2015Article
- Recent progress in understanding the role of ecdysteroids in adult insects: Germline development and circadian clock in the fruit fly Drosophila melanogaster.Zoological letters · 2015Review
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
Abstract
Transcriptional/translational feedback loops drive daily cycles of expression in clock genes and clock-controlled genes, which ultimately underlie many of the overt circadian rhythms manifested by organisms. Moreover, phosphorylation of clock proteins plays crucial roles in the temporal regulation of clock protein activity, stability and subcellular localization. dCLOCK (dCLK), the master transcription factor driving cyclical gene expression and the rate-limiting component in the Drosophila circadian clock, undergoes daily changes in phosphorylation. However, the physiological role of dCLK phosphorylation is not clear. Using a Drosophila tissue culture system, we identified multiple phosphorylation sites on dCLK. Expression of a mutated version of dCLK where all the mapped phospho-sites were switched to alanine (dCLK-15A) rescues the arrythmicity of Clk(out) flies, yet with an approximately 1.5 hr shorter period. The dCLK-15A protein attains substantially higher levels in flies compared to the control situation, and also appears to have enhanced transcriptional activity, consistent with the observed higher peak values and amplitudes in the mRNA rhythms of several core clock genes. Surprisingly, the clock-controlled daily activity rhythm in dCLK-15A expressing flies does not synchronize properly to daily temperature cycles, although there is no defect in aligning to light/dark cycles. Our findings suggest a novel role for clock protein phosphorylation in governing the relative strengths of entraining modalities by adjusting the dynamics of circadian gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.