ArticleGastroenterology and hepatology from bed to bench2014
Molecular identification of Giardia lamblia; is there any correlation between diarrhea and genotyping in Iranian population?
Article in Gastroenterology and hepatology from bed to bench, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Antibiotic resistance patterns, toxin profiles, and toxin gene expression levels among Clostridioides difficile PCR ribotypes isolated from patients scheduled for colonoscopy.Molecular biology reports · 2025Article
- Occurrence and assemblage distribution of Giardia Duodenalis in symptomatic and asymptomatic patients in southeastern Iran (2019-2022).Gut pathogens · 2024Article
- Nitric oxide in parasitic infections: a friend or foe?Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Review
- Assessment of colon cancer molecular mechanism: a system biology approach.Gastroenterology and hepatology from bed to bench · 2021Article
- Intestinal parasitic infections and associated factors in children of three rural schools in Colombia. A cross-sectional study.PloS one · 2019Article
- Giardia lamblia infection: review of current diagnostic strategies.Gastroenterology and hepatology from bed to bench · 2019Review
- Assemblages A and B of Giardia duodenalis reduce enteric glial cells in the small intestine in mice.Parasitology research · 2018Article
- The high frequency of esophageal disorders in Iranian patients with non-cardiac chest pain.Gastroenterology and hepatology from bed to bench · 2018Article
- Evaluation of IL-12A, IL-12B, IL-23A and IL-27 mRNA expression level genes in peripheral mononuclear cells of inflammatory bowel disease patients in an Iranian population.Gastroenterology and hepatology from bed to bench · 2018Article
- Variants in two gene members of the TNF ligand superfamily and hepatitis C virus chronic disease.Gastroenterology and hepatology from bed to bench · 2018Article
- Immunocompromised patients with pulmonary tuberculosis; a susceptible group to intestinal parasites.Gastroenterology and hepatology from bed to bench · 2018Article
- Prevalence of binary-toxin genes (Gastroenterology and hepatology from bed to bench · 2018Article
- Comparison and evaluation of four methods for extracting DNA fromJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017Article
- Nested PCR targeting intergenic spacer (IGS) in genotyping of Giardia duodenalis isolated from symptomatic and asymptomatic infected Egyptian school children.Parasitology research · 2017Article
- First genetic characterisation of Giardia in human isolates from Jordan.Parasitology research · 2016Article
- Disruptions of Host Immunity and Inflammation by Giardia Duodenalis: Potential Consequences for Co-Infections in the Gastro-Intestinal Tract.Pathogens (Basel, Switzerland) · 2015Review
- Advances in understanding Giardia: determinants and mechanisms of chronic sequelae.F1000prime reports · 2015Review
- Genotype Characteristics ofIranian journal of parasitologyArticle
- Anti-protozoal potential of electrospun polymeric nanofiber composite membranes for treatment of contaminated drinking water.Pathogens and global healthArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimThe aim of this study is to investigate the molecular identification of Giardia lamblia in patients with diarrhea.
backgroundGiardiasis caused by Giardia lamblia is a common intestinal disease. Although this parasitic infection found in mammals including human, pets and livestock, but few species within the genus Giardia can infects humans. G. lamblia have seven complex genotypes termed (A-H). Genotype A and B the main causes of human infections. PATIENTS AND
methodsSixty seven microscopically positive G. Lamblia samples were collected from clinical laboratories in Isfahan province between June 2013 and February 2014. Extraction of genomic DNA was performed for 65 concentrated cysts and 2 cultured trophozoites. Partial sequences of tpi including 148-bp and 81-bp were amplified for detection the genotypes A and B using RFLP- PCR protocol respectively.
resultsPCR results showed that out of 67 patients with giardiasis infection, genotype A (148 bp) was detected in 40 isolates (59.70%) compared to genotype B (81 bp) isolated was detected in 25 isolates (37.31%). Also two isolates (2.98%) had mix infection infected with genotype A and B. By comparing the frequency of genotype A (81.8%) and genotype B (13.6%), we found that genotype A is six times higher prevalence than genotype B in patients with diarrhea.
conclusionWe suggest that using sensitive techniques and larger sample for detection of G. lamblia genotypes and their subtypes would be necessary for investigation the immune system respond and correlation with diarrhea in the future studies in Iran.
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25120898PMC4129568W1592671559What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.