Evidence map›Paper›PMID 25120755›Full record

ArticleInternational journal of clinical and experimental pathology2014

Aldo-keto reductase family 1 member B8 is secreted via non-classical pathway.

Zhenwang Tang, Chenglai Xia, Renbin Huang, Xiaoning Li, Wan-Chun Wang, Wangyuan Guo, Lili Duan, Weihao Luo, Deliang Cao, Di-Xian Luo

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Zhenwang TangDepartment of Cardiology, The First Affiliated Hospital of University of South China Hengyang 421001, Hunan, China ; Hunan Engineering Laboratory of Medical Molecular Diagnosis Technology, Institute of Translational Medicine, Affliated The First People's Hospital of Chenzhou, University of South China Chenzhou 423000, Hunan, China.
Chenglai XiaDepartment of Pharmacy, The Third Affiliated Hospital of Guangzhou Medical College Guangzhou 510150, Guangdong, China.
Renbin HuangHunan Engineering Laboratory of Medical Molecular Diagnosis Technology, Institute of Translational Medicine, Affliated The First People's Hospital of Chenzhou, University of South China Chenzhou 423000, Hunan, China.
Xiaoning LiDepartment of Medical Microbiology, Immunology & Cell Biology, Simmons Cooper Cancer Institute, Southern Illinois University School of Medicine 913 N. Rutledge Street, Springfield, IL 62702, USA ; Department of Orthopaedics, The Second Xiangya Hospital of Central South University Middle Ren-Min Road No. 139, Changsha, Hunan 410011, China.
Wan-Chun WangDepartment of Orthopaedics, The Second Xiangya Hospital of Central South University Middle Ren-Min Road No. 139, Changsha, Hunan 410011, China.
Wangyuan GuoHunan Engineering Laboratory of Medical Molecular Diagnosis Technology, Institute of Translational Medicine, Affliated The First People's Hospital of Chenzhou, University of South China Chenzhou 423000, Hunan, China.
Lili DuanHunan Engineering Laboratory of Medical Molecular Diagnosis Technology, Institute of Translational Medicine, Affliated The First People's Hospital of Chenzhou, University of South China Chenzhou 423000, Hunan, China.
Weihao LuoHunan Engineering Laboratory of Medical Molecular Diagnosis Technology, Institute of Translational Medicine, Affliated The First People's Hospital of Chenzhou, University of South China Chenzhou 423000, Hunan, China.
Deliang CaoDepartment of Medical Microbiology, Immunology & Cell Biology, Simmons Cooper Cancer Institute, Southern Illinois University School of Medicine 913 N. Rutledge Street, Springfield, IL 62702, USA.
Di-Xian LuoHunan Engineering Laboratory of Medical Molecular Diagnosis Technology, Institute of Translational Medicine, Affliated The First People's Hospital of Chenzhou, University of South China Chenzhou 423000, Hunan, China.
Chenzhou First People's Hospital · CNCentral South University · CNFirst Affiliated Hospital of University of South China · CNSouthern Illinois University School of Medicine · USThird Affiliated Hospital of Guangzhou Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mouse aldo-keto reductase family 1 member B8 (AKR1B8) has the highest similarity to human aldo-keto reductase family 1 member B10 (AKR1B10), a secretory protein through lysosomes-mediated non-classical secretory pathway. To identify whether AKR1B8 is secreted through the same pathway, we carried out this study. Self-developed sandwich ELISA and western blot were used to detect AKR1B8 in cells and culture medium of CT-26 murine colon carcinoma cells. AKR1B8 releases in an independent manner to Brefeldin A, an inhibitor of ER-to-Golgi classical secretion pathway. Several factors, which are involved in the non-classical secretion pathway, such as temperature, ATP and calcium ion, regulated AKR1B8 secretion from mouse colorectal cancer cells CT-26. Lysosomotropic NH4Cl increased AKR1B8 secretion, and AKR1B8 was located in isolated lysosomes. Therefore, AKR1B8 is a new secretory protein through the lysosomes-mediated non-classical pathway.

Indexed as

Aldehyde ReductaseAldo-Keto ReductasesAnimalsBlotting, WesternCell Line, TumorColonic NeoplasmsEnzyme-Linked Immunosorbent AssayLysosomesMiceRecombinant ProteinsAKR7A5 protein, mouseAldehyde ReductaseAldo-Keto ReductasesRecombinant ProteinsAKR1B8Aldose reductasealdose reductase family 1 member B8non-classical pathwaysecretion

Identifiers

PMID25120755
PMCPMC4128990
OpenAlexW1475083171

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.