ArticleFrontiers in physiology2014
Comparison of two mouse ameloblast-like cell lines for enamel-specific gene expression.
Article in Frontiers in physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 47 citations in OpenAlex.
- Spatial Regulation of Preameloblast Development in Response to Distal Mouse Incisor Injury.Calcified tissue international · 2026Article
- A splicing-derived microRNA from amelogenin exon4 regulates enamel formation via control of exon4 splicing and amelogenin expression.Scientific reports · 2026Article
- A novelFrontiers in physiology · 2026Article
- Notch2 Deletion Compromises Epithelial Integrity and Enamel Formation in Rodent Incisors.Cells · 2025Article
- Next-Generation Strategies for Enamel Repair and Regeneration: Advances in Biomaterials and Translational Challenges.Tissue engineering and regenerative medicine · 2025Review
- Expression ofInternational journal of molecular sciences · 2025Article
- KDF1 promotes ameloblast differentiation by inhibiting the IKK/IκB/NF-κB axis.Journal of cellular physiology · 2024Article
- An unexpected role of neurite outgrowth inhibitor A as regulator of tooth enamel formation.International journal of oral science · 2024Article
- Ameloblastin and its multifunctionality in amelogenesis: A review.Matrix biology : journal of the International Society for Matrix Biology · 2024Review
- From Pluripotent Stem Cells to Organoids and Bioprinting: Recent Advances in Dental Epithelium and Ameloblast Models to Study Tooth Biology and Regeneration.Stem cell reviews and reports · 2024Review
- Research progress of biomimetic materials in oral medicine.Journal of biological engineering · 2023Review
- Injectable hydrogel for sustained delivery of progranulin derivative Atsttrin in treating diabetic fracture healing.Biomaterials · 2023Article
- Organoids from mouse molar and incisor as new tools to study tooth-specific biology and development.Stem cell reports · 2023Article
- Advances in epidemiological status and pathogenesis of dental fluorosis.Frontiers in cell and developmental biology · 2023Review
- Overexpression ofCells · 2022Article
- Coemergence of the Amphipathic Helix on Ameloblastin With Mammalian Prismatic Enamel.Molecular biology and evolution · 2022Article
- Data from ameloblast cell lines cultured in 3D using various gel substrates in the presence of ameloblastin.Data in brief · 2022Article
- Identification of the C-terminal region in Amelogenesis Imperfecta causative protein WDR72 required for Golgi localization.Scientific reports · 2022Article
- WDR72 regulates vesicle trafficking in ameloblasts.Scientific reports · 2022Article
- Mitochondria modulate ameloblast CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Ameloblasts are ectoderm-derived cells that produce an extracellular enamel matrix that mineralizes to form enamel. The development and use of immortalized cell lines, with a stable phenotype, is an important contribution to biological studies as it allows for the investigation of molecular activities without the continuous need for animals. In this study we compare the expression profiles of enamel-specific genes in two mouse derived ameloblast-like cell lines: LS8 and ALC cells. Quantitative PCR analysis indicates that, relative to each other, LS8 cells express greater mRNA levels for genes that define secretory-stage activities (Amelx, Ambn, Enam, and Mmp20), while ALC express greater mRNA levels for genes that define maturation-stage activities (Odam and Klk4). Western blot analyses show that Amelx, Ambn, and Odam proteins are detectable in ALC, but not LS8 cells. Unstimulated ALC cells form calcified nodules, while LS8 cells do not. These data provide greater insight as to the suitability of both cell lines to contribute to biological studies on enamel formation and biomineralization, and highlight some of the strengths and weaknesses when relying on enamel epithelial organ-derived cell lines to study molecular activities of amelogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.