ArticleNucleic acids research2014
Rolling circle replication requires single-stranded DNA binding protein to avoid termination and production of double-stranded DNA.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Volumetric DNA microscopy for mapping spatial transcriptomes in three dimensions.Nature protocols · 2026Review
- Strategies and considerations for the generation of ssDNA-Based HDR templates for CRISPR-based genome editing.BMC genomics · 2026Review
- CLAE: A High-Fidelity Nanopore Sequencing Strategy for Read-Level Viral Variant Detection and Environmental RNA Virus Discovery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Computational analysis of super-resolved in situ sequencing data reveals genes modified by immune-tumor contact events.RNA (New York, N.Y.) · 2024Article
- Direct dsRNA preparation by promoter-free RCT and RNase H cleavage using one circular dsDNA template with a mismatched bubble.RNA (New York, N.Y.) · 2023Article
- A novel isothermal method for amplification of long specific amplicon from linear template.Scientific reports · 2022Article
- AR gene rearrangement analysis in liquid biopsies reveals heterogeneity in lethal prostate cancer.Endocrine-related cancer · 2021Article
- Herpes simplex virus co-infection facilitates rolling circle replication of the adeno-associated virus genome.PLoS pathogens · 2021Article
- DNA flowerstructure co-localizes with human pathogens in infected macrophages.Nucleic acids research · 2020Article
- Dynamics of DNA nicking and unwinding by the RepC-PcrA complex.Nucleic acids research · 2020Article
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- High-Fidelity Nanopore Sequencing of Ultra-Short DNA Targets.Analytical chemistry · 2019Article
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- Preparation and characterization of manganese, cobalt and zinc DNA nanoflowers with tuneable morphology, DNA content and size.Nucleic acids research · 2018Article
- CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window.Nature · 2018Article
- Unveiling the mystery of mitochondrial DNA replication in yeasts.Mitochondrion · 2018Review
- BtsCI and BseGI display sequence preference in the nucleotides flanking the recognition sequence.PloS one · 2018Article
- Entirely enzymatic nanofabrication of DNA-protein conjugates.Nucleic acids research · 2017Article
- New nucleic acid testing devices to diagnose infectious diseases in resource-limited settings.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In rolling circle replication, a circular template of DNA is replicated as a long single-stranded DNA concatamer that spools off when a strand displacing polymerase traverses the circular template. The current view is that this type of replication can only produce single-stranded DNA, because the only 3'-ends available are the ones being replicated along the circular templates. In contrast to this view, we find that rolling circle replication in vitro generates large amounts of double stranded DNA and that the production of single-stranded DNA terminates after some time. These properties can be suppressed by adding single-stranded DNA-binding proteins to the reaction. We conclude that a model in which the polymerase switches templates to the already produced single-stranded DNA, with an exponential distribution of template switching, can explain the observed data. From this, we also provide an estimate value of the switching rate constant.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.