ArticlePloS one2014
An easy and efficient method for native and immunoreactive Echinococcus granulosus antigen 5 enrichment from hydatid cyst fluid.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Human and camel cystic echinococcosis - a polyclonal antibody-based sandwich ELISA for its serodiagnosis with molecular identification.Veterinary research communications · 2024Article
- Comparison of Native Hydatid Cyst Fluid (HCF), Lyophilized HCF, Antigen B (AgB) and Lyophilized AgB (LAgB) Originated fromIranian journal of public health · 2023Article
- IEg67 kDa Bovine Hydatid Cyst Antigen: A Candidate for Developing Sero-Diagnostic Assays for Cystic Echinococcosis, a Disease of One Health Importance.Animals : an open access journal from MDPI · 2023Article
- Expression and production of protoscolex recombinant P29 protein and its serological evaluation for diagnosis of human hydatidosis.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- A simple and efficient purification method of native immunoreactive antigen for diagnosis of camel hydatidosis.Veterinary world · 2020Article
- Liver proteome dataset ofData in brief · 2019Article
- Characterization of paucibacillary ileal lesions in sheep with subclinical active infection by Mycobacterium avium subsp. paratuberculosis.Veterinary research · 2018Article
- Structural and Immunodiagnostic Characterization of Synthetic Antigen B Subunits From Echinococcus granulosus and Their Evaluation as Target Antigens for Cyst Viability Assessment.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Article
- Molecular and biochemical characterization of calmodulin from Echinococcus granulosus.Parasites & vectors · 2017Article
- Comparative Proteomic Analysis ofFrontiers in microbiology · 2017Article
- Proteomic dataset of Paracentrotus lividus gonads of different sexes and at different maturation stages.Data in brief · 2016Article
- Diagnostic Accuracy of Antigen 5-Based ELISAs for Human Cystic Echinococcosis.PLoS neglected tropical diseases · 2016Article
- Immunodiagnosis of human hydatid disease: Where do we stand?World journal of methodology · 2015Review
- Serological Diagnosis and Follow-Up of Human Cystic Echinococcosis: A New Hope for the Future?BioMed research international · 2015Review
- Evaluation of the Diagnostic Performance of Recombinant Antigen B1 for Detection of Cystic Echinococcosis Using Lateral Flow Dipstick Test.Iranian journal of parasitologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCurrently, the serodiagnosis of cystic echinococcosis relies mostly on crude Echinococcus granulosus hydatid cyst fluid as the antigen. Consequently, available immunodiagnostic tests lack standardization of the target antigen and, in turn, this is reflected on poor sensitivity and specificity of the serological diagnosis. METHODOLOGY/PRINCIPAL
findingsHere, a chromatographic method enabling the generation of highly enriched Antigen 5 (Ag5) is described. The procedure is very easy, efficient and reproducible, since different hydatid cyst fluid (HCF) sources produced very similar chromatograms, notwithstanding the clearly evident and extreme heterogeneity of the starting material. In addition, the performance of the antigen preparation in immunological assays was preliminarily assessed by western immunoblotting and ELISA on a limited panel of cystic echinococcosis patients and healthy controls. Following western immunoblotting and ELISA experiments, a high reactivity of patient sera was seen, with unambiguous and highly specific results. CONCLUSIONS/SIGNIFICANCE: The methods and results reported open interesting perspectives for the development of sensitive diagnostic tools to enable the timely and unambiguous detection of cystic echinococcosis antibodies in patient sera.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.