Artificially constructed quorum-sensing circuits are used for subtle control of bacterial population density.

Zhaoshou Wang, Xin Wu, Jianghai Peng, Yidan Hu, Baishan Fang, Shiyang Huang

Open access · goldAbstract read

In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it

0cells of the map it votes in

6citing papers in PubMed

0.9field-weighted citation impact, top 28% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

Metataxonomic and metabolomic evidence of biofilm homeostasis disruption related to caries: An in vitro study.Molecular oral microbiology · 2022
Article
Quantifying the optimal strategy of population control of quorum sensing network in Escherichia coli.NPJ systems biology and applications · 2021
Article
CRAGE-mediated insertion of fluorescent chromosomal markers for accurate and scalable measurement of co-culture dynamics inSynthetic biology (Oxford, England) · 2020
Article
Transcriptional control of motility enables directional movement of Escherichia coli in a signal gradient.Scientific reports · 2017
Article
Synthetic Microbial Ecology: Engineering Habitats for Modular Consortia.Frontiers in microbiology · 2017
Article
Artificial cell-cell communication as an emerging tool in synthetic biology applications.Journal of biological engineering · 2015
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Zhaoshou WangInstitute of Biochemical Engineering, Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, China.

Xin WuInstitute of Biochemical Engineering, Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, China.

Jianghai PengInstitute of Biochemical Engineering, Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

Yidan HuInstitute of Biochemical Engineering, Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, China.

Baishan FangInstitute of Biochemical Engineering, Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China; The Key Lab for Chemical Biology of Fujian Province, Xiamen University, Xiamen, China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, China.

Shiyang HuangInstitute of Biochemical Engineering, Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, China.

Xiamen University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vibrio fischeri is a typical quorum-sensing bacterium for which lux box, luxR, and luxI have been identified as the key elements involved in quorum sensing. To decode the quorum-sensing mechanism, an artificially constructed cell-cell communication system has been built. In brief, the system expresses several programmed cell-death BioBricks and quorum-sensing genes driven by the promoters lux pR and PlacO-1 in Escherichia coli cells. Their transformation and expression was confirmed by gel electrophoresis and sequencing. To evaluate its performance, viable cell numbers at various time periods were investigated. Our results showed that bacteria expressing killer proteins corresponding to ribosome binding site efficiency of 0.07, 0.3, 0.6, or 1.0 successfully sensed each other in a population-dependent manner and communicated with each other to subtly control their population density. This was also validated using a proposed simple mathematical model.

Indexed as

Models, BiologicalAliivibrio fischeriBinding SitesCell DeathDNA PrimersPlasmidsPolymerase Chain ReactionPopulation DensityQuorum SensingDNA Primers

Identifiers

PMID25119347

DOI10.1371/journal.pone.0104578

PMCPMC4132116

OpenAlexW2002674747

What OpenQuestion holds

Textmetadata

LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.