ArticlePloS one2014
Transcriptome analysis of red swamp crawfish Procambarus clarkii reveals genes involved in gonadal development.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Imagin: An Integrase-Like Gene Conserved Across Malacostracan Crustaceans Derived From a Ginger1 DNA Transposon.Genome biology and evolution · 2026Article
- Direct RNA Sequencing Reveals Sex-Biased Transcriptomic and Epitranscriptomic Regulation inBiology · 2025Article
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- Special Issue: Molecular Advance on Reproduction and Fertility of Aquatic Animals.International journal of molecular sciences · 2024Article
- Dietary Threonine Promoted the Growth and Ovarian Development of the Red Swamp Crayfish (Aquaculture nutrition · 2024Article
- Article
- Comparison ofFrontiers in microbiology · 2023Article
- Two Short Repeats in the 5' Untranslated Region of Insulin-like Androgenic Gland Factor inInternational journal of molecular sciences · 2022Article
- Comparative transcriptomic analysis of the different developmental stages of ovary in red swamp crayfish Procambarus clarkii.BMC genomics · 2021Article
- Molecular Targets and Associated Signaling Pathways of Jingshu Granules in Ovarian Cysts Based on Systemic Pharmacological Analysis.BioMed research international · 2021Article
- Validation of Real-time PCR Reference Genes of Muscle Metabolism in Harvested Spiny-Cheek Crayfish (Animals : an open access journal from MDPI · 2020Article
- De novo gonad transcriptome analysis of the common littoral shrimp Palaemon serratus: novel insights into sex-related genes.BMC genomics · 2019Article
- Genome survey sequencing of red swamp crayfish Procambarus clarkii.Molecular biology reports · 2018Article
- Transcriptional Identification of Related Proteins in the Immune System of the CrayfishHigh-throughput · 2018Article
- Investigating the genetic and epigenetic basis of big biological questions with the parthenogenetic marbled crayfish: A review and perspectives.Journal of biosciences · 2018Review
- Sex and tissue specific gene expression patterns identified following de novo transcriptomic analysis of the Norway lobster, Nephrops norvegicus.BMC genomics · 2017Article
- Transcriptomic analysis of the differentiating ovary of the protogynous ricefield eel Monopterus albus.BMC genomics · 2017Article
- In-depth comparative transcriptome analysis of intestines of red swamp crayfish, Procambarus clarkii, infected with WSSV.Scientific reports · 2016Article
- The nucleic acid-binding protein PcCNBP is transcriptionally regulated during the immune response in red swamp crayfish Procambarus clarkii.Cell stress & chaperones · 2016Article
- Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe red swamp crawfish, Procambarus clarkii, has become one of the most economically important cultured species in China. Currently, little is known about the gonadal development of this species. Isolation and characterization of genes are an initial step towards understanding gonadal development of P. clarkii.
resultsUsing the 454 pyrosequencing technology, we obtained a total of 1,134,993 high quality sequence reads from the crawfish testis and ovary libraries. We aimed to identify different genes with a potential role in gonad development. The assembly formed into 22,652 isotigs, distributed by GO analysis across 55 categories in the three ontologies, 'molecular function', 'cellular component', and 'biological processes'. Comparative transcript analysis showed that 1,720 isotigs in the ovary were up-regulated and 2138 isotigs were down-regulated. Several gonad development related genes, such as vitellogenin, cyclin B, cyclin-dependent kinases 2, Dmc1 and ubiquitin were identified. Quantitative real-time PCR verified the expression profiles of 14 differentially expressed genes, and confirmed the reliability of the 454 pyrosequencing.
conclusionsOur findings provide an archive for future research on gonadal development at a molecular level in P. clarkii and other crustacean. This data will be helpful to develop new ideas for artificial regulation of the reproductive process in crawfish aquaculture.
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