ArticlePloS one2014
Successful non-surgical deep uterine transfer of porcine morulae after 24 hour culture in a chemically defined medium.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
- Evaluation of an Ipsilateral Uterine Horn Resection and Ovariectomy Surgical Model in Gilts for Embryo Collection.Animals : an open access journal from MDPI · 2025Article
- Insights into the single-cell transcriptome characteristics of porcine endometrium with embryo loss.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Cytokine profile in peripheral blood mononuclear cells differs between embryo donor and potential recipient sows.Frontiers in veterinary science · 2024Article
- The Use of a Brief Synchronization Treatment after Weaning, Combined with Superovulation, Has Moderate Effects on the Gene Expression of Surviving Pig Blastocysts.Animals : an open access journal from MDPI · 2023Article
- Combined synchronization and superovulation treatments negatively impact embryo viability possibly by the downregulation of WNT/β-catenin and Notch signaling genes in the porcine endometrium.Journal of animal science · 2022Article
- Immunological uterine response to pig embryos before and during implantation.Reproduction in domestic animals = Zuchthygiene · 2022Review
- One-Step In Vitro Generation of ETV2-Null Pig Embryos.Animals : an open access journal from MDPI · 2022Article
- Unveiling how vitrification affects the porcine blastocyst: clues from a transcriptomic study.Journal of animal science and biotechnology · 2022Article
- The Open Cryotop System Is Effective for the Simultaneous Vitrification of a Large Number of Porcine Embryos at Different Developmental Stages.Frontiers in veterinary science · 2022Article
- Transcriptional Profiling of Porcine Blastocysts Produced In Vitro in a Chemically Defined Culture Medium.Animals : an open access journal from MDPI · 2021Article
- Reproductive fluids, used for the in vitro production of pig embryos, result in healthy offspring and avoid aberrant placental expression of PEG3 and LUM.Journal of animal science and biotechnology · 2021Article
- Effects of Vitrification on the Blastocyst Gene Expression Profile in a Porcine Model.International journal of molecular sciences · 2021Article
- A Short-Term Altrenogest Treatment Post-weaning Followed by Superovulation Reduces Pregnancy Rates and Embryo Production Efficiency in Multiparous Sows.Frontiers in veterinary science · 2021Article
- Vitrification Effects on the Transcriptome ofFrontiers in veterinary science · 2021Article
- N-(2-mercaptopropionyl)-glycine enhances in vitro pig embryo production and reduces oxidative stress.Scientific reports · 2020Article
- Boar seminal plasma: current insights on its potential role for assisted reproductive technologies in swine.Animal reproduction · 2020Review
- Pig Pregnancies after Transfer of Allogeneic Embryos Show a Dysregulated Endometrial/Placental Cytokine Balance: A Novel Clue for Embryo Death?Biomolecules · 2020Article
- Allogeneic Embryos Disregulate Leukemia Inhibitory Factor (LIF) and Its Receptor in the Porcine Endometrium During Implantation.Frontiers in veterinary science · 2020Article
- Prevention of hatching of porcine morulae and blastocysts by liquid storage at 20 °C.Scientific reports · 2019Article
- Seminal Plasma Modifies the Transcriptional Pattern of the Endometrium and Advances Embryo Development in Pigs.Frontiers in veterinary science · 2019Article
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Excellent fertility and prolificacy have been reported after non-surgical deep uterine transfers of fresh in vivo-derived porcine embryos. Unfortunately, when this technology is used with vitrified embryos, the reproductive performance of recipients is low. For this reason and because the embryos must be stored until they are transferred to the recipient farms, we evaluated the potential application of non-surgical deep uterine transfers with in vivo-derived morulae cultured for 24 h in liquid stage. In Experiment 1, two temperatures (25 °C and 37 °C) and two media (one fully defined and one semi-defined) were assessed. Morulae cultured in culture medium supplemented with bovine serum albumin and fetal calf serum at 38.5 °C in 5% CO2 in air were used as controls. Irrespective of medium, the embryo viability after 24 h of culture was negatively affected (P<0.05) at 25 °C but not at 37 °C compared with the controls. Embryo development was delayed in all experimental groups compared with the control group (P<0.001). Most of the embryos (95.7%) cultured at 37 °C achieved the full or expanded blastocyst stage, and unlike the controls, none of them hatched at the end of culture. In Experiment 2, 785 morulae were cultured in the defined medium at 37 °C for 24 h, and the resulting blastocysts were transferred to the recipients (n = 24). Uncultured embryos collected at the blastocyst stage (n = 750) were directly transferred to the recipients and used as controls (n = 25). No differences in farrowing rates (91.7% and 92.0%) or litter sizes (9.0 ± 0.6 and 9.4 ± 0.8) were observed between the groups. This study demonstrated, for the first time, that high reproductive performance can be achieved after non-surgical deep uterine transfers with short-term cultured morulae in a defined medium, which opens new possibilities for the sanitary, safe national and international trade of porcine embryos and the commercial use of embryo transfer in pigs.
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