ArticlePloS one2014
A comprehensive reference transcriptome resource for the common house spider Parasteatoda tepidariorum.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 69 citations in OpenAlex.
- Genome-wide quantitative dissection of an arthropod segmented body plan at single-cell resolution.Communications biology · 2025Article
- A taxon-restricted duplicate of Iroquois3 is required for patterning the spider waist.PLoS biology · 2024Article
- De novo assembly of transcriptomes and differential gene expression analysis using short-read data from emerging model organisms - a brief guide.Frontiers in zoology · 2024Review
- An atlas of spider development at single-cell resolution provides new insights into arthropod embryogenesis.EvoDevo · 2024Article
- Female mating rates and their fitness consequences in the common house spiderEcology and evolution · 2022Article
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- The organizing role of Wnt signaling pathway during arthropod posterior growth.Frontiers in cell and developmental biology · 2022Review
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- Systemic paralogy and function of retinal determination network homologs in arachnids.BMC genomics · 2020Article
- Hedgehog signaling controls segmentation dynamics and diversity viaScience advances · 2020Article
- Candidate gene screen for potential interaction partners and regulatory targets of the Hox gene labial in the spider Parasteatoda tepidariorum.Development genes and evolution · 2020Article
- Experimental duplication of bilaterian body axes in spider embryos: Holm's organizer and self-regulation of embryonic fields.Development genes and evolution · 2020Review
- six3 acts upstream of foxQ2 in labrum and neural development in the spider Parasteatoda tepidariorum.Development genes and evolution · 2020Article
- Ovarian Transcriptomic Analyses in the Urban Human Health Pest, the Western Black Widow Spider.Genes · 2020Article
- The common house spiderEvoDevo · 2020Review
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- An overview of Phoneutria nigriventer spider venom using combined transcriptomic and proteomic approaches.PloS one · 2018Article
- Expression and function of the zinc finger transcription factor Sp6-9 in the spider Parasteatoda tepidariorum.Development genes and evolution · 2017Article
- The house spider genome reveals an ancient whole-genome duplication during arachnid evolution.BMC biology · 2017Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Parasteatoda tepidariorum is an increasingly popular model for the study of spider development and the evolution of development more broadly. However, fully understanding the regulation and evolution of P. tepidariorum development in comparison to other animals requires a genomic perspective. Although research on P. tepidariorum has provided major new insights, gene analysis to date has been limited to candidate gene approaches. Furthermore, the few available EST collections are based on embryonic transcripts, which have not been systematically annotated and are unlikely to contain transcripts specific to post-embryonic stages of development. We therefore generated cDNA from pooled embryos representing all described embryonic stages, as well as post-embryonic stages including nymphs, larvae and adults, and using Illumina HiSeq technology obtained a total of 625,076,514 100-bp paired end reads. We combined these data with 24,360 ESTs available in GenBank, and 1,040,006 reads newly generated from 454 pyrosequencing of a mixed-stage embryo cDNA library. The combined sequence data were assembled using a custom de novo assembly strategy designed to optimize assembly product length, number of predicted transcripts, and proportion of raw reads incorporated into the assembly. The de novo assembly generated 446,427 contigs with an N50 of 1,875 bp. These sequences obtained 62,799 unique BLAST hits against the NCBI non-redundant protein data base, including putative orthologs to 8,917 Drosophila melanogaster genes based on best reciprocal BLAST hit identity compared with the D. melanogaster proteome. Finally, we explored the utility of the transcriptome for RNA-Seq studies, and showed that this resource can be used as a mapping scaffold to detect differential gene expression in different cDNA libraries. This resource will therefore provide a platform for future genomic, gene expression and functional approaches using P. tepidariorum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.