ArticleThe Journal of biological chemistry2014
The T4 phage DNA mimic protein Arn inhibits the DNA binding activity of the bacterial histone-like protein H-NS.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 42 citations in OpenAlex.
- Phage-encoded sRNA counteracts xenogeneic silencing in pathogenic E. coli.PLoS pathogens · 2026Article
- Genomic characterization and comparative analysis of a new Tequatrovirus phage infecting Escherichia coli ST131.BMC research notes · 2026Article
- A methylome-derived mbioRxiv : the preprint server for biology · 2026Article
- Biology of host-dependent restriction-modification in prokaryotes.EcoSal Plus · 2025Review
- Post-translational modifications of the nucleoid protein H-NS: sites, mechanisms, and regulatory cues.FEMS microbiology reviews · 2025Review
- Article
- Bacterial chromatin proteins, transcription, and DNA topology: Inseparable partners in the control of gene expression.Molecular microbiology · 2024Review
- Inhibitors of bacterial immune systems: discovery, mechanisms and applications.Nature reviews. Genetics · 2024Review
- Architecture and infection-sensing mechanism of the bacterial PARIS defense system.bioRxiv : the preprint server for biology · 2024Article
- Bacteriophage strategies for overcoming host antiviral immunity.Frontiers in microbiology · 2023Review
- Phages and their satellites encode hotspots of antiviral systems.Cell host & microbe · 2022Article
- Hypermodified DNA in Viruses of E. coli and Salmonella.EcoSal Plus · 2021Review
- Isolation, phenotypic characterization and comparative genomic analysis of 2019SD1, a polyvalent enterobacteria phage.Scientific reports · 2021Article
- Novel anti-repression mechanism of H-NS proteins by a phage protein.Nucleic acids research · 2021Article
- Characterization of the Chimeric PriB-SSBc Protein.International journal of molecular sciences · 2021Article
- A phage-encoded nucleoid associated protein compacts both host and phage DNA and derepresses H-NS silencing.Nucleic acids research · 2021Article
- Overexpression of the Bacteriophage T4Viruses · 2021Article
- Structural basis of transcription inhibition by the DNA mimic protein Ocr of bacteriophage T7.eLife · 2020Article
- Ancestral zinc-finger bearing protein MucR in alpha-proteobacteria: A novel xenogeneic silencer?Computational and structural biotechnology journal · 2020Review
- The architects of bacterial DNA bridges: a structurally and functionally conserved family of proteins.Open biology · 2019Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The T4 phage protein Arn (Anti restriction nuclease) was identified as an inhibitor of the restriction enzyme McrBC. However, until now its molecular mechanism remained unclear. In the present study we used structural approaches to investigate biological properties of Arn. A structural analysis of Arn revealed that its shape and negative charge distribution are similar to dsDNA, suggesting that this protein could act as a DNA mimic. In a subsequent proteomic analysis, we found that the bacterial histone-like protein H-NS interacts with Arn, implying a new function. An electrophoretic mobility shift assay showed that Arn prevents H-NS from binding to the Escherichia coli hns and T4 p8.1 promoters. In vitro gene expression and electron microscopy analyses also indicated that Arn counteracts the gene-silencing effect of H-NS on a reporter gene. Because McrBC and H-NS both participate in the host defense system, our findings suggest that T4 Arn might knock down these mechanisms using its DNA mimicking properties.
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