ArticlePloS one2014
Tetrandrine suppresses lipopolysaccharide-induced microglial activation by inhibiting NF-κB and ERK signaling pathways in BV2 cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- Polypeptides Isolated fromInternational journal of molecular sciences · 2022Article
- Medicinal Plants in the Treatment of Hypertension: A Review.Advanced pharmaceutical bulletin · 2021Review
- Ganoderic Acid A Attenuates LPS-Induced Neuroinflammation in BV2 Microglia by Activating Farnesoid X Receptor.Neurochemical research · 2021Article
- Role of glia in optic nerve.Progress in retinal and eye research · 2021Review
- Phyllanthus amarus prevents LPS-mediated BV2 microglial activation via MyD88 and NF-κB signaling pathways.BMC complementary medicine and therapies · 2020Article
- Anticancer Activity of Tetrandrine by Inducing Apoptosis in Human Breast Cancer Cell Line MDA-MB-231 In Vivo.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- Cascade Signals of Papaverine Inhibiting LPS-Induced Retinal Microglial Activation.Journal of molecular neuroscience : MN · 2019Article
- Article
- The small molecule CA140 inhibits the neuroinflammatory response in wild-type mice and a mouse model of AD.Journal of neuroinflammation · 2018Article
- Modulation of three key innate immune pathways for the most common retinal degenerative diseases.EMBO molecular medicine · 2018Review
- Transcorneal Electrical Stimulation Inhibits Retinal Microglial Activation and Enhances Retinal Ganglion Cell Survival After Acute Ocular Hypertensive Injury.Translational vision science & technology · 2018Article
- Galectin-3 and Galectin-9 May Differently Regulate the Expressions of Microglial M1/M2 Markers and T Helper 1/Th2 Cytokines in the Brains of Genetically Susceptible C57BL/6 and Resistant BALB/c Mice Following Peroral Infection WithFrontiers in immunology · 2018Article
- The role of microglia in the progression of glaucomatous neurodegeneration- a review.International journal of ophthalmology · 2018Review
- An Aqueous Extract of Herbal Medicine ALWPs Enhances Cognitive Performance and Inhibits LPS-Induced Neuroinflammation via FAK/NF-κB Signaling Pathways.Frontiers in aging neuroscience · 2018Article
- Globular Adiponectin Limits Microglia Pro-Inflammatory Phenotype through an AdipoR1/NF-κB Signaling Pathway.Frontiers in cellular neuroscience · 2017Article
- Tetrandrine identified in a small molecule screen to activate mesenchymal stem cells for enhanced immunomodulation.Scientific reports · 2016Article
- Echinocystic Acid Inhibits IL-1β-Induced COX-2 and iNOS Expression in Human Osteoarthritis Chondrocytes.Inflammation · 2016Article
- A novel tetrandrine-loaded chitosan microsphere: characterization and in vivo evaluation.Drug design, development and therapy · 2016Article
- Anti-Hypertensive Herbs and Their Mechanisms of Action: Part II.Frontiers in pharmacology · 2016Review
- Papaverine inhibits lipopolysaccharide-induced microglial activation by suppressing NF-κB signaling pathway.Drug design, development and therapy · 2016Article
Corrections and comments
- Retraction · 2019-10-31Duplication of/in Image · Manipulation of Images ·
- Retracted
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectiveTetrandrine (TET) is a bisbenzylisoquinoline alkaloid extracted from Stephania tetrandra Moore. Recent studies have suggested that TET can reduce the inflammatory response in microglia, but the mechanisms remain unclear. The aim of this study is to investigate whether TET can inhibit lipopolysaccharide (LPS)-induced microglial activation and clarify its possible mechanisms. STUDY DESIGN/MATERIALS AND
methodsCell viability assays and cell apoptosis assays were used to determine the working concentrations of TET. Then, BV2 cells were seeded and pretreated with TET for 2 h. LPS was then added and incubated for an additional 24 hours. qRT-PCR and ELISA were used to measure the mRNA or protein levels of IL1β and TNFα. Western blotting was utilized to quantify the expression of CD11b and cell signaling proteins.
resultsTET at optimal concentrations (0.1 µM, 0.5 µM or 1 µM) did not affect the cell viability. After TET pretreatment, the levels of IL1β and TNFα (both in transcription and translation) were significantly inhibited in a dose-dependent manner. Further studies indicated that phospho-p65, phospho-IKK, and phospho-ERK 1/2 expression were also suppressed by TET.
conclusionsOur results indicate that TET can effectively suppress microglial activation and inhibit the production of IL1β and TNFα by regulating the NF-kB and ERK signaling pathways. Together with our previous studies, we suggest that TET would be a promising candidate to effectively suppress overactivated microglia and alleviate neurodegeneration in glaucoma.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.