ArticlePlanta2014
Cotyledonary somatic embryos of Pinus pinaster Ait. most closely resemble fresh, maturing cotyledonary zygotic embryos: biological, carbohydrate and proteomic analyses.
Article in Planta, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed.
- The 26S proteasome engages in priming explants with auxin plus cytokinin for somatic embryogenesis of Coffea canephora.Protoplasma · 2026Article
- Ectomycorrhizal Symbiosis as a Bio-Enhancement Strategy for Transplantation of Somatic Embryo-DerivedPlants (Basel, Switzerland) · 2026Article
- A transcriptome atlas of zygotic and somatic embryogenesis in Norway spruce.The Plant journal : for cell and molecular biology · 2024Article
- Proteomic Profiling ofInternational journal of molecular sciences · 2024Article
- Article
- Accumulated effects of factors determining plant development from somatic embryos ofFrontiers in plant science · 2022Article
- Desiccation as a Post-maturation Treatment Helps Complete Maturation of Norway Spruce Somatic Embryos: Carbohydrates, Phytohormones and Proteomic Status.Frontiers in plant science · 2022Article
- Constitutive Overexpression of a ConiferFrontiers in plant science · 2022Article
- Identification of Metabolic Pathways Differentially Regulated in Somatic and Zygotic Embryos of Maritime Pine.Frontiers in plant science · 2022Article
- Current Proteomic and Metabolomic Knowledge of Zygotic and Somatic Embryogenesis in Plants.International journal of molecular sciences · 2021Review
- Proteome-Wide Analysis of Heat-Stress inFrontiers in plant science · 2021Article
- Transcriptional analysis of arogenate dehydratase genes identifies a link between phenylalanine biosynthesis and lignin biosynthesis.Journal of experimental botany · 2020Article
- Small RNA profiling in Pinus pinaster reveals the transcriptome of developing seeds and highlights differences between zygotic and somatic embryos.Scientific reports · 2019Article
- Somatic Embryogenesis in Selected Conifer TreesFrontiers in plant science · 2019Review
- Somatic Embryogenesis Induction in Woody Species: The Future After OMICs Data Assessment.Frontiers in plant science · 2019Review
- New Approaches to Optimize Somatic Embryogenesis in Maritime Pine.Frontiers in plant science · 2019Article
- Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.].BMC plant biology · 2018Article
- Advanced Proteomic Approaches to Elucidate Somatic Embryogenesis.Frontiers in plant science · 2018Review
- Carbohydrate-mediated responses during zygotic and early somatic embryogenesis in the endangered conifer, Araucaria angustifolia.PloS one · 2017Article
- Norway spruce embryogenesis: changes in carbohydrate profile, structural development and response to polyethylene glycol.Tree physiology · 2016Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cotyledonary somatic embryos (SEs) of maritime pine are routinely matured for 12 weeks before being germinated and converted to plantlets. Although regeneration success is highly dependent on SEs quality, the date of harvesting is currently determined mainly on the basis of morphological features. This empirical method does not provide any accurate information about embryo quality with respect to storage compounds (proteins, carbohydrates). We first analyzed SEs matured for 10, 12 and 14 weeks by carrying out biological (dry weight, water content) and biochemical measurements (total protein and carbohydrate contents). No difference could be found between collection dates, suggesting that harvesting SEs after 12 weeks is appropriate. Cotyledonary SEs were then compared to various stages, from fresh to fully desiccated, in the development of cotyledonary zygotic embryos (ZEs). We identified profiles that were similar using hierarchical ascendant cluster analysis (HCA). Fresh and dehydrated ZEs could be distinguished, and SEs clustered with fresh ZEs. Both types of embryo exhibited similar carbohydrate and protein contents and signatures. This high level of similarity (94.5 %) was further supported by proteome profiling. Highly expressed proteins included storage, stress-related, late embryogenesis abundant and energy metabolism proteins. By comparing overexpressed proteins in developing and cotyledonary SEs or ZEs, some (23 proteins) could be identified as candidate biomarkers for the late, cotyledonary stage. This is the first report of useful generic protein markers for monitoring embryo development in maritime pine. Our results also suggest that improvements of SEs quality may be achieved if the current maturation conditions are refined.
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