ArticleBMC biology2014
Brachyury cooperates with Wnt/β-catenin signalling to elicit primitive-streak-like behaviour in differentiating mouse embryonic stem cells.
Article in BMC biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Post-transcriptional regulation of Profilin-2 by microRNAs and RNA-binding proteins forms a critical regulatory node for early embryonic cell fate decisions.PLoS genetics · 2026Article
- TGFβ signaling is required during human and chick Neural Crest formation.Developmental biology · 2026Article
- A blastoporal organizer in a ctenophore.Nature · 2026Article
- Investigation of embryogenesis in a dish: the development and application of gastruloid models from pluripotent stem cells.Cell & bioscience · 2026Review
- Optimization of Gastruloid Pre-culture Conditions for Reproducible Germ Layer Composition.Methods in molecular biology (Clifton, N.J.) · 2026Article
- N2B27 media formulations influence gastruloid development.Development (Cambridge, England) · 2025Article
- Coordinate post-transcriptional regulation by microRNAs and RNA binding proteins is critical for early embryonic cell fate decisions.bioRxiv : the preprint server for biology · 2025Article
- Signaling Centers Drive Brachyury Dynamics and Lineage Commitment in mESC Aggregates.FASEB bioAdvances · 2025Article
- Domain-specific AI segmentation of IMPDH2 rod/ring structures in mouse embryonic stem cells.BMC biology · 2025Article
- Stem cell culture conditions affect in vitro differentiation potential and mouse gastruloid formation.PloS one · 2025Article
- Three-dimensional stem cell models of mammalian gastrulation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Early autonomous patterning of the anteroposterior axis in gastruloids.Development (Cambridge, England) · 2024Article
- Basal delamination during mouse gastrulation primes pluripotent cells for differentiation.Developmental cell · 2024Article
- The Nucleosome Remodelling and Deacetylation complex coordinates the transcriptional response to lineage commitment in pluripotent cells.Biology open · 2024Article
- Brachyury co-operates with polycomb protein RYBP to regulate gastrulation and axial elongationFrontiers in cell and developmental biology · 2024Article
- Optimizing Nodal, Wnt and BMP signaling pathways for robust and efficient differentiation of human induced pluripotent stem cells to intermediate mesoderm cells.Frontiers in cell and developmental biology · 2024Article
- Optogenetic control of Wnt signaling models cell-intrinsic embryogenic patterning using 2D human pluripotent stem cell culture.Development (Cambridge, England) · 2023Article
- Spatial transcriptomic profiling to identify mesoderm progenitors with precision genomic screening and functional confirmation.Cell proliferation · 2022Article
- Gastruloids: Pluripotent stem cell models of mammalian gastrulation and embryo engineering.Developmental biology · 2022Article
- Cell-state transitions and collective cell movement generate an endoderm-like region in gastruloids.eLife · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe formation of the primitive streak is the first visible sign of gastrulation, the process by which the three germ layers are formed from a single epithelium during early development. Embryonic stem cells (ESCs) provide a good system for understanding the molecular and cellular events associated with these processes. Previous work, both in embryos and in culture, has shown how converging signals from both nodal/TGFβR and Wnt/β-catenin signalling pathways specify cells to adopt a primitive-streak-like fate and direct them to undertake an epithelial-to-mesenchymal transition (EMT). However, many of these approaches have relied on genetic analyses without taking into account the temporal progression of events within single cells. In addition, it is still unclear to what extent events in the embryo are able to be reproduced in culture.
resultsHere, we combine flow cytometry and a quantitative live single-cell imaging approach to demonstrate how the controlled differentiation of mouse ESCs towards a primitive streak fate in culture results in cells displaying many of the characteristics observed during early mouse development including transient brachyury expression, EMT and increased motility. We also find that the EMT initiates the process, and this is both fuelled and terminated by the action of brachyury, whose expression is dependent on the EMT and β-catenin activity.
conclusionsAs a consequence of our analysis, we propose that a major output of brachyury expression is in controlling the velocity of the cells that are transiting out of the primitive streak.
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