ArticleCirculation research2014
Adipose stromal cells differentiate along a smooth muscle lineage pathway upon endothelial cell contact via induction of activin A.
Article in Circulation research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 59 citations in OpenAlex.
- Activin a regulates vascular formation and stabilization in direct coculture of dental pulp stem cells and endothelial cells.International endodontic journal · 2025Article
- Impaired Communication through Gap Junctions Reduces the Angiogenic Potential of the Secretome in Mesenchymal Stromal Cell-Endothelial Cell Interactions In Vitro.Bulletin of experimental biology and medicine · 2024Article
- Notch Pathways Regulate Expression of Angiostatic Factor Activin A in Endothelial-Pericyte-Like Mesenchymal Stromal Cell Interactions.Stem cells and development · 2023Article
- Adipose tissue-derived stem cells: a comparative review on isolation, culture, and differentiation methods.Cell and tissue banking · 2022Review
- DPSCs treated by TGF-β1 regulate angiogenic sprouting of three-dimensionally co-cultured HUVECs and DPSCs through VEGF-Ang-Tie2 signaling.Stem cell research & therapy · 2021Article
- Transcriptome analysis and functional identification of adipose-derived mesenchymal stem cells in secondary lymphedema.Gland surgery · 2020Article
- Efficient differentiation of vascular smooth muscle cells from Wharton's Jelly mesenchymal stromal cells using human platelet lysate: A potential cell source for small blood vessel engineering.World journal of stem cells · 2020Article
- Lung Microvascular Niche, Repair, and Engineering.Frontiers in bioengineering and biotechnology · 2020Review
- Pro-angiogenic Activity Discriminates Human Adipose-Derived Stromal Cells From Retinal Pericytes: Considerations for Cell-Based Therapy of Diabetic Retinopathy.Frontiers in cell and developmental biology · 2020Article
- CD44 expression in stem cells and niche microglia/macrophages following ischemic stroke.Stem cell investigation · 2020Article
- Review
- TGF-β Secretion by M2 Macrophages Induces Glial Scar Formation by Activating Astrocytes In Vitro.Journal of molecular neuroscience : MN · 2019Article
- miR-27a regulates vascular remodeling by targeting endothelial cells' apoptosis and interaction with vascular smooth muscle cells in aortic dissection.Theranostics · 2019Article
- High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy.Nature communications · 2018Article
- Reversible secretome and signaling defects in diabetic mesenchymal stem cells from peripheral arterial disease patients.Journal of vascular surgery · 2018Article
- Endothelial cells enhance adipose mesenchymal stromal cell-mediated matrix contraction via ALK receptors and reduced follistatin: Potential role of endothelial cells in skin fibrosis.Journal of cellular physiology · 2018Article
- The Adipose Stromal Vascular Fraction as a Complex Cellular Source for Tissue Engineering Applications.Tissue engineering. Part B, Reviews · 2018Review
- Cigarette Smoking Impairs Adipose Stromal Cell Vasculogenic Activity and Abrogates Potency to Ameliorate Ischemia.Stem cells (Dayton, Ohio) · 2018Article
- The Effects of a Pulsed Electromagnetic Field on the Proliferation and Osteogenic Differentiation of Human Adipose-Derived Stem Cells.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Microvascular Networks From Endothelial Cells and Mesenchymal Stromal Cells From Adipose Tissue and Bone Marrow: A Comparison.Frontiers in bioengineering and biotechnology · 2018Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
rationaleAdipose stromal cells (ASC) are therapeutically potent progenitor cells that possess properties of pericytes. In vivo, ASC in combination with endothelial cells (EC) establish functional multilayer vessels, in which ASC form the outer vessel layer and differentiate into mural cells.
objectiveTo identify factors responsible for ASC differentiation toward the smooth muscle cell phenotype via interaction with EC. METHODS AND
resultsAn in vitro model of EC cocultivation with ASC was used, in which EC organized into vascular cords, accompanied by ASC migration toward EC and upregulation of α-smooth muscle actin, SM22α, and calponin expression. Conditioned media from EC-ASC, but not from EC cultures, induced smooth muscle cell protein expression in ASC monocultures. EC-ASC cocultivation induced marked accumulation of activin A but not transforming growth factor-β1 in conditioned media. This was attributed to induction of activin A expression in ASC on contact with EC. Although transforming growth factor-β and activin A were individually sufficient to initiate expression of smooth muscle cell antigens in ASC, only activin A IgG blocked the effect of EC-ASC conditioned media. Although transforming growth factor-β was able to induce activin A expression in ASC, in cocultures this induction was transforming growth factor-β independent. In EC-ASC cocultures, activin A IgG or ALK4/5/7 receptor inhibitors blocked expression of α-smooth muscle actin in ASC in the absence of direct EC-cord contact, but this inhibition was circumvented in ASC by direct EC contact.
conclusionsEC initiate a smooth muscle cell differentiation program in adjacent ASC and propagate this differentiation in distant ASC by induction of activin A expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.