ArticleScientific reports2014
Fixed-target protein serial microcrystallography with an x-ray free electron laser.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
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Who cites it
110 citing papers in PubMed.
- A simple and practical approach for protein serial crystallography using grease matrix and large-area support film.Journal of synchrotron radiation · 2026Article
- Polyethylene Terephthalate Film-Based Sample Holder for Fixed-Target Serial Femtosecond Crystallography.Materials (Basel, Switzerland) · 2026Article
- Automated serial electron diffraction: implementation inJournal of applied crystallography · 2026Article
- Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026Review
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Article
- Macromolecular crystallography and biology at the Linac Coherent Light Source.Journal of synchrotron radiation · 2025Article
- Novel fixed-target serial crystallography flip-holder for macromolecular crystallography beamlines at synchrotron radiation sources.Journal of synchrotron radiation · 2025Article
- Scalable fabrication of an array-type fixed-target device for automated room temperature X-ray protein crystallography.Scientific reports · 2025Article
- Effect of crystal-to-detector distance shift on data processing in serial crystallography.PloS one · 2025Article
- Cyclic Olefin Copolymer-Based Fixed-Target Sample Delivery Device for Protein X-ray Crystallography.Analytical chemistry · 2024Article
- Article
- Article
- Serial X-ray liquidography: multi-dimensional assay framework for exploring biomolecular structural dynamics with microgram quantities.Nature communications · 2024Article
- Power Density Titration of Reversible Photoisomerization of a Fluorescent Protein Chromophore in the Presence of Thermally Driven Barrier Crossing Shown by Quantitative Millisecond Serial Synchrotron X-ray Crystallography.Journal of the American Chemical Society · 2024Article
- Structural biology in the age of X-ray free-electron lasers and exascale computing.Current opinion in structural biology · 2024Review
- Article
- Advanced manufacturing provides tailor-made solutions for crystallography with x-ray free-electron lasers.Structural dynamics (Melville, N.Y.) · 2024Article
- A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers.Nature communications · 2023Article
50 more citing papers are in PubMed but not listed here.
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Authors and funding
16 authors.
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No grant is acknowledged in the PubMed record.
Abstract
We present results from experiments at the Linac Coherent Light Source (LCLS) demonstrating that serial femtosecond crystallography (SFX) can be performed to high resolution (~2.5 Å) using protein microcrystals deposited on an ultra-thin silicon nitride membrane and embedded in a preservation medium at room temperature. Data can be acquired at a high acquisition rate using x-ray free electron laser sources to overcome radiation damage, while sample consumption is dramatically reduced compared to flowing jet methods. We achieved a peak data acquisition rate of 10 Hz with a hit rate of ~38%, indicating that a complete data set could be acquired in about one 12-hour LCLS shift using the setup described here, or in even less time using hardware optimized for fixed target SFX. This demonstration opens the door to ultra low sample consumption SFX using the technique of diffraction-before-destruction on proteins that exist in only small quantities and/or do not produce the copious quantities of microcrystals required for flowing jet methods.
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