ArticleJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry2014
Fluoride inhibition of Sporosarcina pasteurii urease: structure and thermodynamics.
Article in Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 72 citations in OpenAlex.
- Structural landscape of bacterial urease: implications for catalyst and adaptation.Archives of microbiology · 2026Review
- Review
- A constant pH molecular dynamics and experimental study on the effect of different pH on the structure of urease from Sporosarcina pasteurii.Journal of molecular modeling · 2025Article
- Article
- Optimized Ebselen-Based Inhibitors of Bacterial Ureases with Nontypical Mode of Action.Journal of medicinal chemistry · 2023Article
- Inhibition of Urease by Hydroquinones: A Structural and Kinetic Study.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Molecular dynamics simulations, molecular docking, and kinetics study of kaempferol interaction on Jack bean urease: Comparison of extended solvation model.Food science & nutrition · 2022Article
- QM/MM Molecular Dynamics Simulations Revealed Catalytic Mechanism of Urease.The journal of physical chemistry. B · 2022Article
- Assessment of the Ecotoxicity of Pollution by Potentially Toxic Elements by Biological Indicators of Haplic Chernozem of Southern Russia (Rostov region).Water, air, and soil pollution · 2022Article
- Microbial induced calcite precipitation can consolidate martian and lunar regolith simulants.PloS one · 2022Article
- The role of bacterial urease activity on the uniformity of carbonate precipitation profiles of bio-treated coarse sand specimens.Scientific reports · 2021Article
- High-resolution cryo-EM structure of urease from the pathogen Yersinia enterocolitica.Nature communications · 2020Article
- The structure-based reaction mechanism of urease, a nickel dependent enzyme: tale of a long debate.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2020Review
- Remote biosensor for the determination of trypsin by using nanoporous anodic alumina as a three-dimensional nanostructured material.Scientific reports · 2020Article
- Enzyme Kinetics by Isothermal Titration Calorimetry: Allostery, Inhibition, and Dynamics.Frontiers in molecular biosciences · 2020Review
- Inhibition Mechanism of Urease by Au(III) Compounds Unveiled by X-ray Diffraction Analysis.ACS medicinal chemistry letters · 2019Article
- Choosing your (Friedel) mates wisely: grouping data sets to improve anomalous signal.Acta crystallographica. Section D, Structural biology · 2019Article
- Recent advances in design of new urease inhibitors: A review.Journal of advanced research · 2018Review
- Urease-aided calcium carbonate mineralization for engineering applications: A review.Journal of advanced research · 2018Review
- Ureases: Historical aspects, catalytic, and non-catalytic properties - A review.Journal of advanced research · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Urease is a nickel-dependent enzyme and a virulence factor for ureolytic bacterial human pathogens, but it is also necessary to convert urea, the most worldwide used fertilizer, into forms of nitrogen that can be taken up by crop plants. A strategy to control the activity of urease for medical and agricultural applications is to use enzyme inhibitors. Fluoride is a known urease inhibitor, but the structural basis of its mode of inhibition is still undetermined. Here, kinetic studies on the fluoride-induced inhibition of urease from Sporosarcina pasteurii, a widespread and highly ureolytic soil bacterium, were performed using isothermal titration calorimetry and revealed a mixed competitive and uncompetitive mechanism. The pH dependence of the inhibition constants, investigated in the 6.5-8.0 range, reveals a predominant uncompetitive mechanism that increases by increasing the pH, and a lesser competitive inhibition that increases by lowering the pH. Ten crystal structures of the enzyme were independently determined using five crystals of the native form and five crystals of the protein crystallized in the presence of fluoride. The analysis of these structures revealed the presence of two fluoride anions coordinated to the Ni(II) ions in the active site, in terminal and bridging positions. The present study consistently supports an interaction of fluoride with the nickel centers in the urease active site in which one fluoride competitively binds to the Ni(II) ion proposed to coordinate urea in the initial step of the catalytic mechanism, while another fluoride uncompetitively substitutes the Ni(II)-bridging hydroxide, blocking its nucleophilic attack on urea.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.